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Pré-tratamento de resíduos agro-industriais e novas perspectivas na produção de...

Freitas, S. S.; Brazil, O. A. Vital; Nascimento, M. B.; Ferreira, L. F. Romanholo; Gonçalves, A. R.; Michelin, Michele; Polizeli, Maria de Lourdes T. M.

Visando contribuir para a melhoria das condições ambientais pela eliminação dos resíduos agrícolas/agroindustriais, bem como proporcionar a formação de uma linha de pesquisa com propósito de captar e disseminar informações técnicas e tecnológicas agregando maior valor nestes subprodutos ou resíduos é que o presente trabalho foi realizado. Deste modo, o sabugo de milho in natura foi submetido a dois diferentes p...


Autohydrolysis pretreatment of corncob for cellulase production by Trichoderma ...

Michelin, Michele; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Jorge, João A.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.

Microbial cellulase production has attracted great attention due its several applications. In this context, studies that become this practice feasible are very important. Corncob is an inexpensive byproduct, which contains more than 30% of cellulose; however, this material is not readily available to enzymatic hydrolysis and pretreatment of lignocellulosic material in autohydrolysis processes become this more e...


Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch pr...

Michelin, Michele; Mota, A.; Polizeli, Maria de Lourdes T. M.; Silva, Daniel Pereira da; Vicente, A. A.; Teixeira, J. A.

Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (kLa). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of kLa values, in attempts to optimize and compare the activities of extracellular xylanase and β-xylosi...


Effect of oxygen transfer rate on cellulases production in stirred tank and int...

Michelin, Michele; Mota, André; Silva, D. P.; Vicente, A. A.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.


Evaluation of autohydrolysis process for cellulases production by Aspergillus n...

Michelin, Michele; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.


A novel xylan degrading β-D-xylosidase : purification and biochemical character...

Michelin, Michele; Peixoto-Nogueira, Simone C.; Silva, Tony M.; Jorge, João A.; Terenzi, Héctor F.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.

Aspergillus ochraceus, a thermotolerant fungus isolated in Brazil from decomposing materials, produced an extracellular b-xylosidase that was purified using DEAE-cellulose ion exchange chromatography, Sephadex G-100 and Biogel P-60 gel filtration. b-xylosidase is a glycoprotein (39 % carbohydrate content) and has a molecular mass of 137 kDa by SDS-PAGE, with optimal temperature and pH at 70 C and 3.0–5.5, respe...


Xylanase and β-xylosidase production by Aspergillus ochraceus : new perspective...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Jorge, João A.; Terenzi, Héctor F.

The xylanase biosynthesis is induced by its substrate—xylan. The high xylan content in some wastes such as wheat residues (wheat bran and wheat straw) makes them accessible and cheap sources of inducers to be mainly applied in great volumes of fermentation, such as those of industrial bioreactors. Thus, in this work, the main proposal was incorporated in the nutrient medium wheat straw particles decomposed to s...


Production of xylanase and β-xylosidase from autohydrolysis liquor of corncob u...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Ruiz, Héctor A.; Vicente, A. A.; Jorge, João A.

Agroindustrial residues are materials often rich in cellulose and hemicellulose. The use of these substrates for the microbial production of enzymes of industrial interest is mainly due to their high availability associated with their low cost. In this work, corncob (CCs) particles decomposed to soluble compounds (liquor) were incorporated in the microbial growth medium through autohydrolysis, as a strategy to ...


Production of xylanolytic enzymes by Aspergillus terricola in stirred tank and ...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, D. P.; Vicente, A. A.; Jorge, João A.; Terenzi, Héctor F.

Fungi producing high xylanase levels have attracted considerable attention because of their potential industrial applications. Batch cultivations of Aspergillus terricola fungus were evaluated in stirred tank and airlift bioreactors, by using wheat bran particles suspended in the cultivation medium as substrate for xylanase and b-xylosidase production. In the stirred tank bioreactor, in physical conditions of 3...


Pretreatment of brewers' spent grains for cellulases production by Aspergillus ...

Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.; Silva, Daniel Pereira da; Vicente, A. A.; Teixeira, J. A.

Successful utilization of cellulosic materials as a renewable carbon source is dependent on the development of economically feasible process technologies both for the production of biomass-degrading enzymes, and for the enzymatic hydrolysis of cellulosic materials to low molecular weight products. Significant cost reduction is required in order to enhance the commercial viability of cellulase production technol...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia