Author(s):
Negri, M. 
; Henriques, Mariana ; Svidzinski, T. ; Azeredo, Joana ; Oliveira, Rosário
Date: 2013
Persistent ID: http://hdl.handle.net/1822/26055
Origin: RepositóriUM - Universidade do Minho
Document details
Virulence factor of Candida tropicalis isolated from hospitalized patients
Description
Candida tropicalis has been reported to be one of the Candida species which is most likely to cause bloodstream and urinary tract infections in hospitals. Several virulence factors seem to be responsible for C. tropicalis infections, which present high potential for dissemination and mortality. The aim of this study was to investigate the correlation between different virulence factors (enzymes secretion, adhesion and biofilm formation) and antifungal susceptibility of several C. tropicalis clinical isolates. This study was conducted with 8 isolates of C. tropicalis obtained from urine cultures (4), from blood culture (1) and from central venous catheter (1), from patients admitted to intensive care units at the University Hospital in Maringá, Paraná, Brazil. C. tropicalis ATCC 750 was also used, as a control. Virulence factors evaluated included: adhesion to epithelial cells and silicone, biofilm formation and enzyme production (hemolysins, proteinases, and phospholipases). Susceptibility to fluconazole, itraconazole, voriconazole, and amphotericin B was also determined, by E-test. Regarding adhesion, it can be highlighted that C. tropicalis adhered significantly more (p<0.05) to epithelial cells than to silicone. Morevoer, it was verified that all C. tropicalis were prone to form biofilms on silicone. Regarding C. tropicalis enzymatic activity, it was possible to verify that all isolates were able to express total hemolytic activity on sheep-blood agar medium supplemented with glucose. However, proteinase was only produced by two urine isolates and by the isolates from catheter and blood and only one C. tropicalis (from catheter) was phospholipase positive. All isolates were susceptible to voriconazole, fluconazole and amphotericin B. The largest percentage of susceptibility-dose dependence was observed for itraconazole in 4 strains (57.1%). Furthermore one clinical isolate (14.3%) from urine was found to be resistant to the same compound (MIC = 1 µg/ml). Thus, it is possible to conclude that there was no direct correlation between the virulence factors assayed (secretion of enzymes, adhesion to epithelial cells and silicone and biofilm formation). Concerning C. tropicalis susceptibility, it was not possible to establish any relation with Candida virulence factors as well. However, it is important to highlight that all isolates presented one or more virulence factors.
Candida tropicalis has been reported to be one of the Candida species which is most likely to cause bloodstream and urinary tract infections in hospitals. Several virulence factors seem to be responsible for C. tropicalis infections, which present high potential for dissemination and mortality. The aim of this study was to investigate the correlation between different virulence factors (enzymes secretion, adhesion and biofilm formation) and antifungal susceptibility of several C. tropicalis clinical isolates. This study was conducted with 8 isolates of C. tropicalis obtained from urine cultures (4), from blood culture (1) and from central venous catheter (1), from patients admitted to intensive care units at the University Hospital in Maringá, Paraná, Brazil. C. tropicalis ATCC 750 was also used, as a control. Virulence factors evaluated included: adhesion to epithelial cells and silicone, biofilm formation and enzyme production (hemolysins, proteinases, and phospholipases). Susceptibility to fluconazole, itraconazole, voriconazole, and amphotericin B was also determined, by E-test. Regarding adhesion, it can be highlighted that C. tropicalis adhered significantly more (p<0.05) to epithelial cells than to silicone. Morevoer, it was verified that all C. tropicalis were prone to form biofilms on silicone. Regarding C. tropicalis enzymatic activity, it was possible to verify that all isolates were able to express total hemolytic activity on sheep-blood agar medium supplemented with glucose. However, proteinase was only produced by two urine isolates and by the isolates from catheter and blood and only one C. tropicalis (from catheter) was phospholipase positive. All isolates were susceptible to voriconazole, fluconazole and amphotericin B. The largest percentage of susceptibility-dose dependence was observed for itraconazole in 4 strains (57.1%). Furthermore one clinical isolate (14.3%) from urine was found to be resistant to the same compound (MIC = 1 µg/ml). Thus, it is possible to conclude that there was no direct correlation between the virulence factors assayed (secretion of enzymes, adhesion to epithelial cells and silicone and biofilm formation). Concerning C. tropicalis susceptibility, it was not possible to establish any relation with Candida virulence factors as well. However, it is important to highlight that all isolates presented one or more virulence factors.
Document Type
Conference Object
Language English
Language English
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