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Silver colloidal nanoparticles : effect on matrix composition and structure of ...
Monteiro, D. R.; Silva, Sónia Carina; Negri, M.; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, MarianaAim : The aim of this study was to assess the effect of different silver nanoparticles (SN) concentrations on the matrix composition and structure of Candida albicans and Candida glabrata biofilms. Methods and Results : Candida biofilms were developed in 6-well microtiter plates during 48 h. After, these biofilms were exposed to 13·5 or 54 μg SN ml−1 for 24 h. Then, extracellular matrices were extracted from bi...
Virulence factor of Candida tropicalis isolated from hospitalized patients
Negri, M.; Henriques, Mariana; Svidzinski, T.; Azeredo, Joana; Oliveira, RosárioCandida tropicalis has been reported to be one of the Candida species which is most likely to cause bloodstream and urinary tract infections in hospitals. Several virulence factors seem to be responsible for C. tropicalis infections, which present high potential for dissemination and mortality. The aim of this study was to investigate the correlation between different virulence factors (enzymes secretion, adhes...
Silver nanoparticles : influence of stabilizing agent and diameter on antifunga...
Monteiro, D. R.; Silva, Sónia Carina; Negri, M.; Gorup, L. F.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D. B.; Henriques, MarianaAim : The purpose of this work was to evaluate the size-dependent antifungal activity of different silver nanoparticles (SN) colloidal suspensions against Candida albicans and Candida glabrata mature biofilms. Methods and Results: The research presented herein used SN of three different average sizes (5, 10 and 60 nm), which were synthesized by the reduction of silver nitrate through sodium citrate and which w...
Candida tropicalis biofilms : effect on urinary epithelial cells
Negri, M.; Silva, Sónia Carina; Brêda, D.; Henriques, Mariana; Azeredo, Joana; Oliveira, RosárioCandida tropicalis infection is strongly associated with the presence of biofilms in urinary catheters. Thus, the aim of this work was to study the behaviour of C. tropicalis in biofilms of different ages (24e120 h) formed in artificial urine (AU) and their effect in human urinary bladder cells (TCC-SUP). Reference strain ATCC 750 and two isolates from patients with candiduria (U69 and U75) were used in this st...
Silicone colonization by non-Candida albicans Candida species in the presence o...
Silva, Sónia Carina; Negri, M.; Henriques, Mariana; Oliveira, Rosário; Williams, David; Azeredo, JoanaUrinary tract infections (UTIs) are the most common nosocomial infections and 80 % are related to the use of urinary catheters. Furthermore, Candida species are responsible for around 15 % of UTIs and an increasing involvement of non-Candida albicans Candida (NCAC) species (e.g. Candida glabrata, Candida tropicalis and Candida parapsilosis) has been recognized. Given the fact that silicone is frequently used in...
Insights into Candida tropicalis nosocomial infections and virulence factors
Negri, M.; Silva, Sónia Carina; Henriques, Mariana; Oliveira, RosárioCandida tropicalis is considered the first or the second non-Candida albicans Candida (NCAC) species most frequently isolated from candidosis, mainly in patients admitted in intensive care units (ICUs), especially with cancer, requiring prolonged catheterization, or receiving broad-spectrum antibiotics. The proportion of candiduria and candidemia caused by C. tropicalis varies widely with geographical area and ...
Effect of pretreatment of Candida biofilm cells with silver nanoparticles on th...
Monteiro, D. R.; Negri, M.; Silva, Sónia Carina; Camargo, E. R.; Gorup, L. F.; Oliveira, Rosário; Barbosa, D.; Henriques, MarianaObjectives: The pathogenicity of Candida spp. has been recognized by some factors, including adhesion to host cells or inert surfaces and biofilm formation. In addition, the dispersal of biofilm cells may act as a continuous source of infection. Thus, the aim of this study was to evaluate the adhesion capacity of Candida albicans and Candida glabrata cells recovered from Candida biofilms treated with silver nan...
Virulence factors of non-Candida albicans Candida species
Silva, Sónia Carina; Negri, M.; Monteiro, D. R.; Henriques, Mariana; Oliveira, Rosário; Azeredo, JoanaInfections caused by Candida species (candidosis) have greatly increased over recent years, mainly due to the escalation of the AIDS epidemic, population ageing, increasing number of immunocompromised patients and the more widespread use of indwelling medical devices. Besides Candida albicans, non-Candida albicans Candida (NCAC) species such as Candida glabrata, Candida tropicalis and Candida parapsilosis are n...
Silver colloidal nanoparticles: antifungal effect against Candida albicans and ...
Monteiro, D. R.; Gorup, L. F.; Silva, Sónia Carina; Negri, M.; Camargo, E. R.; Oliveira, Rosário; Barbosa, D.; Henriques, MarianaThe aim of this study was to evaluate the effect of silver nanoparticles (SN) against Candida albicans and Candida glabrata adhered cells and biofilms. SN (average diameter 5 nm) were synthesized by silver nitrate reduction with sodium citrate and stabilized with ammonia. Minimal inhibitory concentration (MIC) tests were performed for C. albicans (n = 2) and C. glabrata (n = 2) grown in suspension following the...
Adherence and biofilm formation of non-Candida albicans Candida species
Silva, Sónia Carina; Negri, M.; Henriques, Mariana; Williams, David; Oliveira, Rosário; Azeredo, JoanaMost cases of candidosis have been attributed to Candida albicans, but recently non-Candida albicans Candida species have been identified as frequent human pathogens. Candida pathogenicity has been attributed to several factors, including adhesion to medical devices and/or host cells, biofilm formation, and secretion of hydrolytic enzymes (proteases, phospholipases, and haemolysins). Although ‘new’ Candida spe...
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