Autor(es):
Coelho, Filipa Alexandra Baltar Lobo 
; Alves, Diana Filipa Barros ; Pereira, M. O.
Data: 2012
Identificador Persistente: http://hdl.handle.net/1822/25686
Origem: RepositóriUM - Universidade do Minho
Assunto(s): Terpinen-4-ol; Antimicrobial peptides; Colistin; Biofilms; Prophylactic strategies; Antimicrobial synergism
Descrição
Due to widespread and indiscriminate use of conventional antibiotics, bacteria have acquired a resistant
phenotype in response to those antimicrobials pressure. Therefore, microbial infections associated with biofilms
have become hard to treat with conventional therapy. The development of microbial drug resistance and drugrelated
toxicity has promoted the search for new alternatives, to control, mostly, healthcare-associated infections.
Terpinen-4-ol is the major component of tea tree essential oil and has shown strong antimicrobial properties as a
single agent against planktonic cultures. Colistin is an antimicrobial peptide with great antimicrobial activity,
essentially against Gram-negative bacteria, such as P. aeruginosa.
The aim of this study was to explore the synergism between terpinen-4-ol and colistin, using low doses of each
natural antimicrobial, to control the establishment of P. aeruginosa biofilms. Biofilms were formed in the
presence of both antmicrobials, alone or in combination, being after characterized by total biomass, through
crystal violet, and number of cultivable bacterial cells (log CFU/cm2).
Data related with the individual antimicrobial activity of terpinen-4-ol revealed that the biomass of P.aeruginosa
biofilms was significantly reduced for 0.19 % (v/v), though it not affected the viability of cells even for the
highest concentration tested (0.38 % (v/v)). On the other hand, colistin promoted a significantly reduction of the
biofilm mass but only for concentrations higher than 1 μg ml-1. The number of viable cells entrapped within the
biofilms was only affect for colistin doses higher for 4 μg ml-1. The association of terpinen-4-ol (0.19 % (v/v))
with colistin revealed to be a very efficient prophylactic strategy, as it impaired significantly biofilm formation.
In fact, it was observed biofilm mass reductions closed to 100 % and significant decreases of the numbers of
viable biofilm-cells (3-5 log of reduction) even for the lowest colistin concentration tested (0.5 μg ml-1).
These data highlighted the promising antibiofilm activity of association of natural compounds, as antimicrobial
peptides and secondary metabolites of essential oils, suggesting that this combination may have prophylactic
potential for the prevention of P. aeruginosa biofilm-associated infections.
