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Antimicrobial pressure of ciprofloxacin and gentamicin on biofilm development b...

This work aims at characterizing endoscope biofilm-isolated (PAI) and 2 reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity 3 to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to 4 intermittent antibiotic pressure [ciprofloxacin (CIP) and gentamicin (GM)], as 5 well as the development of resistance towards antibiotics and benzalkonium 6 chloride (BC), wer...

A new approach to bacterial colony morphotyping by matrix-assisted laser desorp...

Matrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping.To this end,four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria.Results suggest that mass spectrometry of intact bacteria could,in some extent,be used to complement the classical morphological classification ...

Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkoniu...

This study aimed to assess the membrane modifications in Pseudomonas aeruginosa after continuous exposure to increasing doses of benzalkonium chloride (BC). Two different concentrations were used, 0.9 and 12.0 mM. Proteomic investigations revealed that the range of the outermembrane proteome alterations following continuous exposure is very low, i.e. about 10% and BC concentration dependent. Adapted cells revea...

Improvements on colony morphology identification towards bacterial profiling

Colony morphology may be an indicator of phenotypic variation, this being an important adaptive process adopted by bacteria to overcome environmental stressors. Furthermore, alterations in colony traits may reflect increased virulence and antimicrobial resistance. Despite the potential relevance of using colony morphological traits, the influence of experimental conditions on colony morphogenesis has been scarc...

Impact of hypoxic and anaerobic environments on multidrug-resistance of emergin...

OBJECTIVES: It is well-known the establishment of steep oxygen gradients in cystic fibrosis (CF) airways mucus, giving rise to hypoxic or anaerobic zones in the deeper mucus layers, where traditional and atypical bacteria may accommodate and proliferate to biofilms. This study aimed to compare the influence of low-oxygen atmospheres on biofilm growth and susceptibility profiles of the CF-atypical species Inquil...

Biological features of Pseudomonas aeruginosa biofilms : time to rethink antimi...

Bacterial infections in cystic fibrosis (CF) lungs are generally associated with mucoid P. aeruginosa and small colonies variants (SCV). Previous works showed that several other colony variants were isolated from CF lungs showing as well resistance to antibiotics. Those variants until now devaluated may play a relevant role in CF population persistence to antibiotic therapies and immune defences. Phenotypic swi...

A colistin coating to prevent biomaterial-associated infections

Synergistic interactions within polymicrobial biofilms formed by atypical and c...

A complex microbial ecosystem is present in Cystic fibrosis (CF)-airways, with uncommon species co-existing and establishing dynamic interactions with traditional pathogens. This study aimed to examine in vitro biofilm formation and susceptibility patterns of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA), under oxygen-atmospheres resembli...

Small colony variants in biofilm population is not strictly required for biofil...

Pseudomonas aeruginosa is a well-known opportunistic pathogen, responsible for high mortality in several human infections. Such infection success is partly due to its phenotypic plasticity to switch to “fitter” phenotypes, such as small colony variants (SCV), generally associated to increased antibiotic resistance, and biofilm formation within the human body. Once embedded in biofilms, P. aeruginosa can augment...

A systematic approach to the interrogation and sharing of standardised biofilm ...

Publicado em "6th International Conference on Practical Applications of Computational Biology & Bioinformatics", ISBN 978-3-642-28838-8 ; The study of microorganism consortia, also known as biofilms, is associated to a number of applications in biotechnology, ecotechnology and clinical domains. A public repository on existing biofilm studies would aid in the design of new studies as well as promote collaborati...