Osteoinduction in human fat derived stem cells by recombinant human bone morpho...

• Author(s): Bessa, Paulo C. ; Pedro, A. J. ; Klosch, B. ; Nobre, A. ; Griensven, Martijn van ; Reis, R. L. ; Casal, Margarida
• Date: 2008
• Persistent ID: http://hdl.handle.net/1822/14042
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Bone morphogenetic protein-2; Recombinant protein purification; Stem cells; Tissue engineering
• Document Type: Article
• Language: English

Description

Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity.