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Osteoinduction in human fat derived stem cells by recombinant human bone morpho...
Bessa, Paulo C.; Pedro, A. J.; Klosch, B.; Nobre, A.; Griensven, Martijn van; Reis, R. L.; Casal, MargaridaBioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fa...
Data: 2008
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Origem: RepositóriUM - Universidade do Minho
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