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Dynamic culturing of cartilage tissue : the significance of hydrostatic pressure
Correia, Cristina; Pereira, Ana L.; Duarte, Ana Rita C.; Frias, A. M.; Pedro, A. J.; Oliveira, João T.; Sousa, R. A.; Reis, R. L.Human articular cartilage functions under a wide range of mechanical loads in synovial joints, where hydrostatic pressure (HP) is the prevalent actuating force. We hypothesized that the formation of engineered cartilage can be augmented by applying such physiologic stimuli to chondrogenic cells or stem cells, cultured in hydrogels, using custom-designed HP bioreactors. To test this hypothesis, we investigated t...
Development and characterization of a novel hybrid tissue engineering-based sca...
Silva, Nuno; Salgado, A. J.; Sousa, R. A.; Oliveira, João T.; Pedro, A. J.; Almeida, Hugo Leite; Cerqueira, R.; Almeida, Armando; Mastronardi, F.Spinal cord injury (SCI) represents a significant health and social problem, and therefore it is vital to develop novel strategies that can specifically target it. In this context, the objective of the present work was to develop a new range of three-dimensional (3D) tubular structures aimed at inducing the regeneration within SCI sites. Up to six different 3D tubular structures were initially developed by rapi...
Biomimetic Ca-P coatings Incorporating bisphosphonates produced on starch-based...
Oliveira, A. L.; Pedro, A. J.; Saiz-Arroyo, C.; Mano, J. F.; Rodriguez, G.; Roman, J. San; Reis, R. L.In this study, sodium clodronate, a well-known therapeutic agent from the family of bisphosphonates (BPs), is incorporated in a biomimetic calcium phosphate (CaP) coating, previously formed on the surface of a starch-based biomaterial by a sodium silicate methodology, as a strategy to develop a site-specific drug delivery system for bone tissue regeneration applications. The effects on the resulting CaP coating...
Hierarchical starch-based fibrous scaffold for bone tissue engineering applicat...
Martins, A.; Chung, S.; Pedro, A. J.; Sousa, R. A.; Marques, A. P.; Reis, R. L.; Neves, N. M.Fibrous structures mimicking the morphology of the natural extracellular matrix are considered promising scaffolds for tissue engineering. This work aims to develop a novel hierarchical starch-based scaffold. Such scaffolds were obtained by a combination of starch-polycaprolactone micro- and polycaprolactone nano-motifs, respectively produced by rapid prototyping (RP) and electrospinning techniques. Scanning el...
Osteoinduction in human fat derived stem cells by recombinant human bone morpho...
Bessa, Paulo C.; Pedro, A. J.; Klosch, B.; Nobre, A.; Griensven, Martijn van; Reis, R. L.; Casal, MargaridaBioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fa...
Patterning of polymer nanofiber meshes by electrospinning for biomedical applic...
Neves, N. M.; Campos, R.; Pedro, A. J.; Cunha, J.; Macedo, F.; Reis, R. L.The end-product of the electrospinning process is typically a randomly aligned fiber mesh or membrane. This is a result of the electric field generated between the drop of polymer solution at the needle and the collector. The developed electric field causes the stretching of the fibers and their random deposition. By judicious selection of the collector architecture, it is thus possible to develop other morphol...
Adult stem cells in bone and cartilage tissue engineering
Salgado, A. J.; Oliveira, João T.; Pedro, A. J.; Reis, R. L.The progressive increase in lif€ expectancy within the last century has led to the appearance of novel health related problems, some of those within the musculoskeletal Íield. Among the latter, one can find diseases such as osteoporosis, rheumatoid arthritis and bone cancer, just to mention some of the most relevant. Other related problems are those that arise from serious injuries, often leading to non-recover...
Chitosan particles agglomerated scaffolds for cartilage and osteochondral tissu...
Malafaya, P. B.; Pedro, A. J.; Anja, Peterbauer; Gabriel, C.; Redl, Heinz; Reis, R. L.It is well accepted that natural tissue regeneration is unlikely to occur if the cells are not supplied with an extracellular matrix (ECM) substitute. With this goal, several different methodologies have been used to produce a variety of 3D scaffolds as artificial ECM substitutes suitable for bone and cartilage tissue engineering. Furthermore, osteochondral tissue engineering presents new challenges since the ...
Soluble starch and composite starch Bioactive Glass 45S5 particles : synthesis,...
Silva, G. A.; Pedro, A. J.; Costa, F. J.; Neves, N. M.; Coutinho, O. P.; Reis, R. L.For many biomedical applications, biodegradable and simultaneously bioactive materials are desired. These materials should at the same time be able to support cell function and co-exist with the organism without triggering a relevant immune response. In this work, the synthesis as well as the bioactivity evaluation of newly developed polymer soluble potato starch and composite (with Bioactive Glass 45S5) micron...
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