Biological activity of heterologous murine interleukin-10 and preliminary studi...

• Author(s): Carvalho, Vera ; Castanheira, Pedro ; Faria, Tiago Q. ; Gonçalves, Catarina ; Madureira, Pedro ; Faro, Carlos ; Domingues, Lucília ; Brito, Rui M. M. ; Vilanova, Manuel ; Gama, F. M.
• Date: 2010
• Persistent ID: http://hdl.handle.net/1822/11211
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Protein delivery system; Anti-inflammatory cytokine; Interleukin-10; Endotoxin-activated macrophages; Dextrin nanogel
• Document Type: Article
• Language: English

Description

Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer with two intramolecular disulphide bonds essential for its biological activity. A mutated form of murine IL-10 was successfully expressed in E. coli, recovered and purified from inclusion bodies. Its ability to reduce tumor necrosis factor synthesis and down-regulate class II major histocompatibility complex molecules expression on endotoxin-stimulated bone marrow-derived macrophages was confirmed, and shown to be similar to that of a commercially available IL-10. Given the potential of IL-10 for application in various medical conditions, it is essential to develop systems that can effectively deliver the protein. In this work it is shown that a dextrin nanogel effectively incorporate IL-10, stabilize, and enable the slow release of biologically active IL-10 over time. Altogether, these results demonstrate the suitability of dextrin nanogel to be used as a system for the controlled release of IL-10.