Using zeta-potential measurements to quantify peptide partition to lipid membranes

• Autor(es): Freire, João M. ; Domingues, Marco M. ; Matos, Joana ; Melo, Manuel N. ; Veiga, Ana Salomé ; Santos, Nuno C. ; Castanho, Miguel A. R. B.
• Data: 2011
• Identificador Persistente: http://hdl.handle.net/10451/6743
• Origem: Repositório da Universidade de Lisboa
• Assunto(s): Partition constant; Zeta-potential; Fluorescence; Membrane; Antimicrobial peptides
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

© The Author(s) 2011. This article is published with open access at Springerlink.com. Open Access: This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. Many cellular phenomena occur on the biomembranes. There are plenty of molecules (natural or xenobiotics) that interact directly or partially with the cell membrane. Biomolecules, such as several peptides (e.g., antimicrobial peptides) and proteins, exert their effects at the cell membrane level. This feature makes necessary investigating their interactions with lipids to clarify their mechanisms of action and side effects necessary. The determination of molecular lipid/water partition constants (Kp) is frequently used to quantify the extension of the interaction. The determination of this parameter has been achieved by using different methodologies, such as UV-Vis absorption spectrophotometry, fluorescence spectroscopy and ζ-potential measurements. In this work, we derived and tested a mathematical model to determine the Kp from ζ-potential data. The values obtained with this method were compared with those obtained by fluorescence spectroscopy, which is a regular technique used to quantify the interaction of intrinsically fluorescent peptides with selected biomembrane model systems. Two antimicrobial peptides (BP100 and pepR) were evaluated by this new method. The results obtained by this new methodology show that ζ-potential is a powerful technique to quantify peptide/lipid interactions of a wide variety of charged molecules, overcoming some of the limitations inherent to other techniques, such as the need for fluorescent labeling.