Ecosapentanoic acid (EPA) does not affect cell kinetics in peripheral lymphocit...

• Author(s): Brito, Miguel ; Ferreira, Paula ; Guerreiro, Catarina Sousa ; Malta-Vacas, Joana ; Tavares, L. ; Santos, P. ; Pinto, A. ; Cravo, Marília
• Date: 2008
• Persistent ID: http://hdl.handle.net/10400.21/3364
• Origin: Repositório Científico do Instituto Politécnico de Lisboa
• Subject(s): Crohn disease; Ecosapentanoic acid; Genotypes; IL6 174 CC
• Document Type: Conference Object
• Language: English

Description

Rationale: Omega 3 fatty acids have been shown to be of potential benefit in patients with CD. The aim of the present study was to evaluate whether EPA can modulate the inflammatory response according to different genotypes of IL6G174G/C polymorphism. Methods: Peripheral blood cells were collected from CD patients with different genotypes for IL6 174G/C (GG, n = 16, GC, n = 8, CC, n = 7), and lymphocytes were established in culture media. Replicates with the addition of EPA (25 mM) were analysed in a period of 24h, 48h and 72h. Expression of IL6 e a PGE2 was assessed by ELISA. Apoptosis and cellular proliferation was determined by flow cytometry.