Autor(es):
Silva, Marta Barreto da 
; Francisco, Vânia ; Rasteiro, Paula ; Sousa, Eduardo ; Vicente, Astrid ; Bourbon, Mafalda ; Martins, Fátima ; Seixas, Maria Teresa ; Fernandes, Aida ; Beleza, Álvaro ; Mendonça, Francisco ; Gil, Ana Paula ; Dias, Carlos Matias
Data: 2011
Identificador Persistente: http://hdl.handle.net/10400.18/568
Origem: Repositório Científico do Instituto Nacional de Saúde
Assunto(s): Evolutionary and Population Genetics; Gene Environment Interaction; Genetic Epidemiology; Population Genetics; Public Health; Population Structure; Estados de Saúde e de Doença; Doenças Cardio e Cérebro-vasculares
Descrição
Abstrat disponível em: http://www.ichg2011.org/cgi-bin/showdetail.pl?absno=11013 The objective of the National Health Examination Survey (NHES), which corresponds to the Portuguese
component of the European Health Examination Survey (EHES), is to collect health data, related risk factors
and biological samples of the Portuguese population, using the EHES recommended methodology. These
surveys involve an interview, clinical and physical measurements and blood collection. In this context, we
herein describe the pilot study performed in S. Brás de Alportel in the Algarve region. For this pilot study, we
have recruited 221 individuals (95 males and 126 females), between 25 and 91 years old, who were enrolled in
the Health Centre of S. Brás de Alportel (Algarve). For each participant, we have collected 16.5 ml of total
blood, in five different Vacutainer® tubes, which was later processed into serum, plasma and DNA. We have
performed several biochemical analyses(total cholesterol, LDL,HDL, glucose, tryglicerides, creatinine, ALT,
AST, -GT, CRP and iron) and a complete blood count. From the 221 participants in this pilot study, we were
able to collect blood to 219 (99.5%). To 185 of these (84.5%) we were able to collect the total amount of blood.
The biochemical analyses were performed in all the samples. The total blood count was performed in 103
samples (47%) due to transport constraints. We have also collected DNA from 210 participants (95.9%). We
have created a biobank comprising 1847 serum aliquots and 959 plasma aliquots, which have been stored at -
80°C and 210 DNA aliquots which have been stored at 4°C. In conclusion, during this study, we have
optimized the logistics and procedures to perform the large scale study for the NHES and EHES. In addition,
we have created a biobank comprising detailed questionnaire data, physical and clinical data and biological
samples from a representative sample of S. Brás de Alportel in Algarve, Portugal. This biobank will allow us to
perform future studies, including the determination of the prevalence of gene variants of public health interest,
the characterization of gene-environment interactions in the development of chronic diseases and the genetic
structure of the Portuguese population. The success rate, the quality of the data and of the biological samples
was high and comparable to similar studies.
