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Cloning, characterization and in vitro and in planta expression of a glucanase ...

Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamom...

Disposition of [U-2H7]glucose into hepatic glycogen in rat and in seabass

The stimulation of hepatic glycogenesis is a ubiquitous response to a glucose challenge and quantifying its contribution to glucose uptake informs its role in restoring euglycemia. Glycogenesis can be quantified with labeled water provided that exchange of glucose-6-phosphate hydrogen 2 (G6P-H2) and body water via glucose-6-phosphate isomerase, and exchange of positions 4, 5 and 6 hydrogens (G6P-H456) via trans...

Os caminhos da autonomia no Online

A história diz-nos que sempre foi assim, à medida que os avanços tecnológicos surgiam no jornalismo os reflexos faziam-se sentir nas alterações de rotinas, na produção noticiosa, na difusão da informação e nas formas de consumo pelo público. Hoje em dia assistimos a profundas mudanças que se intensificaram com o aparecimento das plataformas digitais. As alterações são tão profundas que proporcionam questionamen...

Facts related to the collection of biological samples in the National Health Ex...

Abstrat disponível em: http://www.ichg2011.org/cgi-bin/showdetail.pl?absno=11013 ; The objective of the National Health Examination Survey (NHES), which corresponds to the Portuguese component of the European Health Examination Survey (EHES), is to collect health data, related risk factors and biological samples of the Portuguese population, using the EHES recommended methodology. These surveys involve an inte...

Characterization of molecular factors from plants pathogen Phytophthora cinnamo...

The culture of the chestnut tree is extremely important in the northern region of Portugal, occupying a significant proportion of useful agricultural area. The annual average chestnut production in Portugal can reach 20 000 tons. New plantation areas have increased in the last few decades. However the ink disease caused by the oomycete Phytophthora cinnamomi has damage and killed many trees and up to now no con...

Isolation and characterization of necrosis-inducing phytophthora protein 1 (npp...

Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. Due to their particular physiological characteristics, no efficient treatments against diseases caused by these microorganisms are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phytoph...

Characterization of molecular factors from plants pathogen Phytophthora cinnamomi

The culture of the chestnut tree is extremely important in the northern region of Portugal, occupying a significant proportion of useful agricultural area. The annual average chestnut production in Portugal can reach 20 000 tons. New plantation areas have increased in the last few decades. However the ink disease caused by the oomycete Phytophthora cinnamomi has damage and killed many trees and up to now no con...

Characterization of a glucanase inhibitor protein from Phytophthora cinnamomi i...

Oomycetes from the genus Phytophthora are fungos-likeplant pathogens that are devastating fr agriculture and natural ecosystems. Due to their partucular physiological characteristics, no eficiente tratmentts, it against diseases caused by these microorganisms are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phy...

Expression analysis by RT-qPCR of NPP1 gene from Phytophthora cinnamomi

Expression analysis by RT-qPCR of NPP1 gene from Phytophthora cinnamomi

Species of the genus Phytophthora secrete glucanase inhibitor proteins (GIPs ) to inhibit the activity of enzymes involved in plant defense responses, including during plant infection process of Castanea sativa by Phytophthora cinnamomi. GIPs show structural homology to the chymotrypsin class of serine proteases (SP) but lack proteolytic activity due to the absence of an intact catalytic triad and, thus, belong...