NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a no...

• Author(s): Pauleta, Sofia R. ; Duarte, Américo G ; Carepo, Marta S. ; Pereira, Alice S. ; Tavares, Pedro ; Moura, Isabel ; Moura, José J. G.
• Date: 2007
• Persistent ID: http://hdl.handle.net/10362/8701
• Origin: Repositório Institucional da UNL
• Subject(s): NMR assignment; Carbon detection; Novel Mo–Cu cluster; Desulfovibrio
• Document Type: Article
• Language: English

Description

Biomol NMR Assign (2007) 1:81–83 DOI 10.1007/s12104-007-9022-3 We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo–Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments.