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New spectroscopic and electrochemical insights on a class I superoxide reductas...
Folgosa, Filipe; Cordas, Cristina M.; Santos, Joana A.; Pereira, Alice S.; Moura, José J. G.; Tavares, Pedro; Moura, IsabelBiochem. J. (2011) 438,485–494 doi:10.1042/BJ20110836
Low-Spin Heme b3 in the Catalytic Center of Nitric Oxide Reductase from Pseudom...
Timóteo, Cristina G.; Pereira, Alice S.; Martins, Carlos E.; Naik, Sunil G.; Duarte, Américo G.; Moura, José J. G.; Tavares, Pedro; Moura, IsabelBiochemistry, 2011, 50 (20), pp 4251–4262 DOI: 10.1021/bi101605p ; Respiratory nitric oxide reductase (NOR) was purified from membrane extract of Pseudomonas (Ps.) nautica cells to homogeneity as judged by polyacrylamide gel electrophoresis. The purified protein is a heterodimer with subunits of molecular masses of 54 and 18 kDa. The gene encoding both subunits was cloned and sequenced. The amino acid sequence...
Camelid nanobodies raised against an integral membrane enzyme, nitric oxide red...
Conrath, Katja; Pereira, Alice S.; Martins, Carlos E.; Timóteo, Cristina G.; Tavares, Pedro; Moura, Isabel; Moura, José J. G.Protein Sci. 2009 Mar;18(3):619-28. doi: 10.1002/pro.69. ; Nitric Oxide Reductase (NOR) is an integral membrane protein performing the reduction of NO to N2O. NOR is composed of two subunits: the large one (NorB) is a bundle of 12 transmembrane helices (TMH). It contains a b type heme and a binuclear iron site, which is believed to be the catalytic site, comprising a heme b and a non-hemic iron. The small subu...
Molybdenum Induces the expression of a protein containing a new heterometallic ...
Rivas, Maria G.; Carepo, Marta S. P.; Mota, Cristiano S.; Moura, José J. G.; Moura, Isabel; Korbas, Malgorzata; Lopes, Ana T.; Brondino, Carlos D.Biochemistry. 2009 Feb 10;48(5):873-82. doi: 10.1021/bi801773t. ; The characterization of a novel Mo-Fe protein (MorP) associated with a system that responds to Mo in Desulfovibrio alaskensis is reported. Biochemical characterization shows that MorP is a periplasmic homomultimer of high molecular weight (260 +/- 13 kDa) consisting of 16-18 monomers of 15321.1 +/- 0.5 Da. The UV/visible absorption spectrum of t...
Electron transfer complex between nitrous oxide reductase and cytochrome c552 f...
Moura, Isabel; Moura, José J. G.; Pauleta, Sofia R; Monzani, Enrico; Pereira, Alice S.; Casella, Luigi; Dell’Acqua, SimoneBiochemistry. 2008 Oct 14;47(41):10852-62. doi: 10.1021/bi801375q ; The multicopper enzyme nitrous oxide reductase (N 2OR) catalyzes the final step of denitrification, the two-electron reduction of N 2O to N 2. This enzyme is a functional homodimer containing two different multicopper sites: CuA and CuZ. CuA is a binuclear copper site that transfers electrons to the tetranuclear copper sulfide CuZ, the catalyt...
NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a no...
Pauleta, Sofia R.; Duarte, Américo G; Carepo, Marta S.; Pereira, Alice S.; Tavares, Pedro; Moura, Isabel; Moura, José J. G.Biomol NMR Assign (2007) 1:81–83 DOI 10.1007/s12104-007-9022-3 ; We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo–Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments.
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