Encontrados 75 documentos, a visualizar página 1 de 8
Probing the surface chemistry of different oxidized MWCNT for the improved elec...
Silveira, Célia M.; Pimpão, Marta; Pedroso, Humberto A.; Rodrigues, Patrícia R. S.; Moura, José J. G.; Pereira, Manuel F. R.; Almeida, M. Gabriela"This work reports the evaluation of a set of multi-walled carbon nanotubes (MWCNT) presenting different surface chemistries, as interfaces for the direct electrochemistry of the multihemic nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC27774 (Dd). The carbon nanotubes dispersions were prepared in aqueous media and deposited on pyrolytic graphite (PG) macroelectrodes, following a layer-by-layer ...
Recent advances into vanadyl, vanadate and decavanadate interactions with actin
Ramos, S.; Moura, José J. G.; Aureliano, M.Metallomics. 2012 Jan 3;4(1):16-22
Recent advances into vanadyl, vanadate and decavanadate interactions with actin
Ramos, Susana; Moura, José J. G.; Aureliano, M.Although the number of papers about ‘‘vanadium’’ has doubled in the last decade, the studies about ‘‘vanadium and actin’’ are scarce. In the present review, the effects of vanadyl, vanadate and decavanadate on actin structure and function are compared. Decavanadate 51V NMR signals, at 516 ppm, broadened and decreased in intensity upon actin titration, whereas no effects were observed for vanadate monomers, at ...
Study of membrane ageing and grafting mechanisms using electron paramagnetic ...
Oliveira, Fábio R. P.; Matos, Cristina T.; Moura, José J. G.; Portugal, Carla A. M.; Crespo, João G.An important setback for a wider use of membrane processes in industry is fouling, caused by aggregation of biomolecules at membrane surface and pores. Two important approaches to reduce this effect are the use of chemical cleaning procedures and the functionalisation of the membrane surface. However, both processes may lead to membrane degradation and structure alteration due to free radical formation or ra...
Gd(III) chelates as NMR probes of protein-protein interactions. Case study: rub...
Almeida, Rui M.; Geraldes, Carlos F. G. C.; Pauleta, Sofia R.; Moura, José J. G.Inorganic Chemistry 50(21):10600-7 ; Two cyclen-derived Gd probes, [Gd-DOTAM](3+) and [Gd-DOTP](5-) (DOTAM = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetamide; DOTP = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylenephosphonate)), were assessed as paramagnetic relaxation enhancement (PRE)-inducing probes for characterization of protein-protein interactions. Two proteins, Desulfovibrio gigas rubre...
The mechanism of formate oxidation by metal-dependent formate dehydrogenases
Mota, Cristiano S.; Rivas, Maria G.; Brondino, Carlos D.; Moura, Isabel; Moura, José J. G.; González, Pablo J.; Cerqueira, Nuno M. F. S. A.J Biol Inorg Chem (2011) 16:1255–1268 DOI 10.1007/s00775-011-0813-8 ; Metal-dependent formate dehydrogenases (Fdh) from prokaryotic organisms are members of the dimethyl sulfoxide reductase family of mononuclear molybdenum-containing and tungsten-containing enzymes. Fdhs catalyze the oxidation of the formate anion to carbon dioxide in a redox reaction that involves the transfer of two electrons from the substr...
New spectroscopic and electrochemical insights on a class I superoxide reductas...
Folgosa, Filipe; Cordas, Cristina M.; Santos, Joana A.; Pereira, Alice S.; Moura, José J. G.; Tavares, Pedro; Moura, IsabelBiochem. J. (2011) 438,485–494 doi:10.1042/BJ20110836
Analysis of the activation mechanism of Pseudomonas stutzeri cytochrome c perox...
Sousa, P. M. Paes de; Rodrigues, D.; Timóteo, C. G.; Gonçalves, M. L. Simões; Pettigrew, G. W.; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2011) 16:881–888 DOI 10.1007/s00775-011-0785-8
Low-Spin Heme b3 in the Catalytic Center of Nitric Oxide Reductase from Pseudom...
Timóteo, Cristina G.; Pereira, Alice S.; Martins, Carlos E.; Naik, Sunil G.; Duarte, Américo G.; Moura, José J. G.; Tavares, Pedro; Moura, IsabelBiochemistry, 2011, 50 (20), pp 4251–4262 DOI: 10.1021/bi101605p ; Respiratory nitric oxide reductase (NOR) was purified from membrane extract of Pseudomonas (Ps.) nautica cells to homogeneity as judged by polyacrylamide gel electrophoresis. The purified protein is a heterodimer with subunits of molecular masses of 54 and 18 kDa. The gene encoding both subunits was cloned and sequenced. The amino acid sequence...
The tetranuclear copper active site of nitrous oxide reductase: the CuZ center
Dell’Acqua, Simone; Pauleta, Sofia R.; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2011) 16:183–194 DOI 10.1007/s00775-011-0753-3 ; This review focuses on the novel CuZ center of nitrous oxide reductase, an important enzyme owing to the environmental significance of the reaction it catalyzes, reduction of nitrous oxide, and the unusual nature of its catalytic center, named CuZ. The structure of the CuZ center, the unique tetranuclear copper center found in this enzyme, ope...
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