Description
β-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application,
mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides.
Several advantages are associated with the application of immobilized enzymes on magnetic
supports. In this work, β-galactosidase was covalently immobilised onto a Polysiloxane-
Polyvinyl Alcohol Magnetic Composite (mPOS-PVA), Magnetic Polysiloxane with Polyaniline
(mPOS-PANI), Magnetized Dacron (DACRON) and Magnetite with Polyaniline (MAG-PANI)
using glutaraldehyde as activating agent being the synthesis of GOS evaluated and
compared at different temperatures (30, 40, 50 and 60 oC) and various initial lactose
concentration (50, 100, 200, 300, 400 and 500 g/L). The kinetic parameters obtained by
fitting the experimental data were compared in order to determine the effect of the
immobilization process with different supports on the synthesis of oligosaccharides. These
results clearly demonstrate that all supports may be used for β-galactosidase immobilization
as, besides improving the enzyme hydrolytic and GOS synthesis properties, its separation
from the obtained reaction products is easy to accomplish.
; Balcão, V. M. 
