Encontrados 6 documentos, a visualizar página 1 de 1
Immobilized β-galactosidase onto magnetic particles coated with polyaniline: Su...
Neri, D. F. M.; Balcão, V. M.; Dourado, Fernando; Oliveira, J. M. B.; Carvalho Junior, L. B.; Teixeira, J. A.Magnetite particles (<100 μm) obtained by coprecipitation of Fe2+ and Fe3+ and coated with polyaniline (MAG-PANI) were used to immobilize Aspergillus oryzae β-galactosidase via glutaraldehyde. The amount of β-galactosidase immobilized onto MAG-PANI was ca. 2.04 mg/g support. This magnetic enzymatic derivative was capable to act on lactose and to produce tri and tetragalactosides (transgalactosylation) and the c...
Characterization of galactooligosaccharides produced by β-galactosidase immobil...
Neri, D. F. M.; Balcão, V. M.; Cardoso, Susana M.; Silva, Artur M. S.; Domingues, M. R. M.; Torres, D.; Rodrigues, L. R.; Carvalho Junior, L. B.Bioconversions using enzymes immobilized in magnetic supports present significant advantages due to the easy separation of the enzyme from the reaction mixture and the simplicity and low cost of the support preparation. The characterization of the oligosaccharide mixture obtained by the action of b-galactosidase covalently attached, via glutaraldehyde, to a hydrazideeDacronemagnetite composite is presented. The...
Galacto-oligosaccharides production during lactose hydrolysis by free Aspergill...
Neri, D. F. M.; Balcão, V. M.; Costa, Rafael S.; Rocha, I.; Ferreira, E. C.; Torres, D.; Rodrigues, L. R.; Carvalho Junior, L. B.; Teixeira, J. A.The synthesis of galacto-oligosaccharides (GOS) by the action of Aspergillus oryzae β-galactosidase free and immobilized on magnetic polysiloxane-polyvinyl alcohol (mPOS-PVA) was studied. A maximum GOS concentration of 26% (w/v) of total sugars was achieved at near 55% lactose conversion from 50%, w/v lactose solution at pH 4.5 and 40 °C. Trisaccharides accounted for more than 81% of the total GOS produced. GOS...
Galactooligosaccharides production by β-galactosidase immobilized onto magnetic...
Neri, D. F. M.; Balcão, V. M.; Dourado, Fernando; Oliveira, J. M.; Carvalho Junior, L. B.; Teixeira, J. A.Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their tim...
Immobilization of b-galactosidase from kluyveromyces lactis onto a polysiloxane...
Neri, D. F. M.; Balcão, V. M.; Carneiro-da-Cunha, Maria G.; Carvalho Junior, L. B.; Teixeira, J. A.β-Galactosidase from Kluyveromyces lactis was covalently immobilized onto a mPOS–PVA, using glutaraldehyde as activating agent and its properties were evaluated. The enzymatic water insoluble derivative displayed the same optimum pH (6.5) and optimum temperature (50 °C) of the soluble enzyme. The apparent Km app and activation energy for both soluble and immobilized enzyme derivative were found to be not signif...
β-Galactosidase from Aspergillus oryzae immobilized onto different magnetic sup...
Neri, D. F. M.; Balcão, V. M.; Costa, Rafael S.; Ferreira, E. C.; Torres, D.; Rodrigues, Lígia M.; Carvalho Junior, L. B.; Teixeira, J. A.β-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes on magnetic supports. In this work, β-galactosidase was covalently immobilised onto a Polysiloxane- Polyvinyl Alcohol Magnetic Composite (mPOS-PVA), Magn...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||