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Nanoencapsulation of bovine lactoferrin for food and biopharmaceutical applicat...

Lactoferrin has for long captured the interest of many researchers as a natural compound with a wide variety of uses. Lactoferrin is a monomeric, iron-binding 80 kDa glycoprotein, and appears to be the subfraction of whey with the best documented antiviral, antimicrobial, anticancer and immune modulating/enhancing effects. It belongs to the family of transferrin proteins, and serves to control iron levels in bo...

Immobilized β-galactosidase onto magnetic particles coated with polyaniline: Su...

Magnetite particles (<100 μm) obtained by coprecipitation of Fe2+ and Fe3+ and coated with polyaniline (MAG-PANI) were used to immobilize Aspergillus oryzae β-galactosidase via glutaraldehyde. The amount of β-galactosidase immobilized onto MAG-PANI was ca. 2.04 mg/g support. This magnetic enzymatic derivative was capable to act on lactose and to produce tri and tetragalactosides (transgalactosylation) and the c...

Characterization of galactooligosaccharides produced by β-galactosidase immobil...

Bioconversions using enzymes immobilized in magnetic supports present significant advantages due to the easy separation of the enzyme from the reaction mixture and the simplicity and low cost of the support preparation. The characterization of the oligosaccharide mixture obtained by the action of b-galactosidase covalently attached, via glutaraldehyde, to a hydrazideeDacronemagnetite composite is presented. The...

Structural and functional stabilization of glycomacropeptide via encapsulation ...

Bovine glycomacropeptide (GMP), derived from whey proteins, has been demonstrated to possess an interesting bioactivity that has attracted a lot of attention over the last few years. In particular, its ability to bind Vibrio cholerae and Escherichia coli enterotoxins, inhibit bacterial and viral adhesion, suppress gastric secretions, promote bifidobacterial growth and modulate immune system responses. Of these,...

Biopolymeric matrices for structural and functional stabilization of bacterioph...

In the recent past years, bacteriophage research has experienced a renaissance due to their potential application in the pharmaceutical field, especially with the increase of bacterial resistance to antibiotics and the possibility to take part in new methods of early detection and diagnosis of bacterial infections. In that context, the structural and functional stabilization of bacteriophages using biopolymeric...

Galacto-oligosaccharides production during lactose hydrolysis by free Aspergill...

The synthesis of galacto-oligosaccharides (GOS) by the action of Aspergillus oryzae β-galactosidase free and immobilized on magnetic polysiloxane-polyvinyl alcohol (mPOS-PVA) was studied. A maximum GOS concentration of 26% (w/v) of total sugars was achieved at near 55% lactose conversion from 50%, w/v lactose solution at pH 4.5 and 40 °C. Trisaccharides accounted for more than 81% of the total GOS produced. GOS...

Galactooligosaccharides production by β-galactosidase immobilized onto magnetic...

Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose concentrations (5–50%) and temperatures (30–60 °C). The initial lactose concentration in the reaction media affected the total amounts of produced GOS and their tim...

Total antioxidant activity and trace elements in human milk : the first 4 month...

The content of many nutrients in breast milk are dependent on the nutritional status of the lactating woman. This is particularly true for fat and water-soluble vitamins, some of which have antioxidant properties. The aim of the study entertained herein was to evaluate the changes in total antioxidant status of human milk during the first 4 months of lactation, and to correlate such changes with the contents in...

A multiple emulsion formulation of bacteriophage encapsulated in lipid nanovesi...

Immobilization of b-galactosidase from kluyveromyces lactis onto a polysiloxane...

β-Galactosidase from Kluyveromyces lactis was covalently immobilized onto a mPOS–PVA, using glutaraldehyde as activating agent and its properties were evaluated. The enzymatic water insoluble derivative displayed the same optimum pH (6.5) and optimum temperature (50 °C) of the soluble enzyme. The apparent Km app and activation energy for both soluble and immobilized enzyme derivative were found to be not signif...