Immobilization of b-galactosidase from kluyveromyces lactis onto a polysiloxane...

• Author(s): Neri, D. F. M. ; Balcão, V. M. ; Carneiro-da-Cunha, Maria G. ; Carvalho Junior, L. B. ; Teixeira, J. A.
• Date: 2008
• Persistent ID: http://hdl.handle.net/1822/8158
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): b-Galactosidase; Kluyveromyces lactis; Covalent immobilization; Polysiloxane; Polyvinyl alcohol; Magnetic support
• Document Type: Article
• Language: English

Description

β-Galactosidase from Kluyveromyces lactis was covalently immobilized onto a mPOS–PVA, using glutaraldehyde as activating agent and its properties were evaluated. The enzymatic water insoluble derivative displayed the same optimum pH (6.5) and optimum temperature (50 °C) of the soluble enzyme. The apparent Km app and activation energy for both soluble and immobilized enzyme derivative were found to be not significantly different. The mPOS–PVA β-galactosidase preparation presented a higher operational and thermal stability than the soluble enzyme. This immobilized β-galactosidase also was effective in hydrolyzing lactose from milk. Hence, one can conclude that mPOS–PVA is an attractive and efficient support for β-galactosidase immobilization.