Author(s):
Simões, M. 
; Simões, Lúcia C. ; Machado, Idalina ; Pereira, M. O. ; Vieira, M. J.
Date: 2006
Persistent ID: http://hdl.handle.net/1822/6061
Origin: RepositóriUM - Universidade do Minho
Subject(s): Biofilm control; Hydrodynamic conditions; Recovery; Resistance; Surfactant
Description
The action of cetyltrimethyl ammonium bromide (CTAB) and sodium dodecyl sulphate
(SDS), a cationic and an anionic surfactant respectively, were investigated for their
ability to control turbulent and laminar flow-generated biofilms formed by P. fluorescens.
The disinfectant action of CTAB and SDS on biofilms was assessed by respiratory activity,
variation of mass and structure, immediately, 3, 7 and 12 h after the application of the surfactants.
Laminar flow-generated biofilms were more susceptible to the action of CTAB than
those formed under turbulent flow. Total inactivation of the cells within the biofilms was
not achieved for either type of flow- enerated biofilms. For SDS, higher concentrations promoted
significant biofilm inactivation, for both turbulent and laminar flow-generated biofilms.
CTAB and SDS application did not promote the detachment of biofilms from the surfaces.
Post-surfactant treatment, biofilms recovered respiratory activity, in some cases, reaching
values higher than those found without chemical treatment. After CTAB treatment, the recovery of respiratory activity was not affected by the hydrodynamic conditions. Conversely, turbulent flow-generated biofilms showed a higher potential to recover their metabolic activity than laminar flow-generated biofilms, when previously challenged with SDS. Concerning biofilm mass, no significant variation (increase or decrease) was detected after 12 h of surfactant treatment. This study shows that care is needed when selecting the correct procedure and agent for biofilm control and demonstrates the influence of hydrodynamic conditions on the persistent and recalcitrant properties of P. fluorescens biofilms.
