Author(s):
Rodrigues, L. R. 
; Moldes, Ana ; Teixeira, J. A. ; Oliveira, Rosário
Date: 2005
Persistent ID: http://hdl.handle.net/1822/3463
Origin: RepositóriUM - Universidade do Minho
Document details
Modeling of biosurfactant production by Lactobacillus Strains
Description
Screening of biosurfactant-producing ability of three Lactobacillus strains was performed, being shown that, for all the tested strains, biosurfactant production is occurring mainly in the first 7 hours. All strains reduced 8 mN/m the surface tension of the fermentation broth at the end of fermentation and lysed blood agar with scores ranging between (++) corresponding to complete hemolysis with a diameter < 1 cm. Time courses of lactose, biomass and biosurfactant were modeled according to reported models, assuming product inhibition. Using optimized MRS broth as culture medium, the values estimated by the modeling of biosurfactant were P_max = 1.4 g of biosurfactant/L and rp/X = 0.137g/L.hˉ¹ for Lactobacillus casei CECT-5275, P_max = 1.5 g/L and rp/X = 0.145g/L.hˉ¹ for Lactobacillus rhamnosus CECT-288 and P_max = 1.5 g/L and rp/X = 0.089 g/L.hˉ¹ for Lactobacillus pentosus CECT-4023. Using whey as production medium, the values obtained for Lactobacillus rhamnosus CECT-288 were P_max = 1.3 g of biosurfactant/L and rp/X = 0.078 g/L.hˉ¹. In conclusion, the results obtained showed that whey can be used as an alternative medium for biosurfactant production by Lactobacillus strains.
Screening of biosurfactant-producing ability of three Lactobacillus strains was performed, being shown that, for all the tested strains, biosurfactant production is occurring mainly in the first 7 hours. All strains reduced 8 mN/m the surface tension of the fermentation broth at the end of fermentation and lysed blood agar with scores ranging between (++) corresponding to complete hemolysis with a diameter < 1 cm. Time courses of lactose, biomass and biosurfactant were modeled according to reported models, assuming product inhibition. Using optimized MRS broth as culture medium, the values estimated by the modeling of biosurfactant were P_max = 1.4 g of biosurfactant/L and rp/X = 0.137g/L.hˉ¹ for Lactobacillus casei CECT-5275, P_max = 1.5 g/L and rp/X = 0.145g/L.hˉ¹ for Lactobacillus rhamnosus CECT-288 and P_max = 1.5 g/L and rp/X = 0.089 g/L.hˉ¹ for Lactobacillus pentosus CECT-4023. Using whey as production medium, the values obtained for Lactobacillus rhamnosus CECT-288 were P_max = 1.3 g of biosurfactant/L and rp/X = 0.078 g/L.hˉ¹. In conclusion, the results obtained showed that whey can be used as an alternative medium for biosurfactant production by Lactobacillus strains.
Document Type
Conference Object
Language English
Language English
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