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An overview of the recent developments on fructooligosaccharide production and ...

Dominguez, Ana; Rodrigues, L. R.; Lima, Nelson; Teixeira, J. A.

Over the past years, many researchers have suggested that deficiencies in the diet can lead to disease states and that some diseases can be avoided through an adequate intake of relevant dietary components. Recently, a great interest in dietary modulation of the human gut has been registered. Prebiotics, such as fructooligosaccharides (FOS), play a key role in the improvement of gut microbiota balance and in in...


Thermal and hydrolytic degradation of electrospun fish gelatin membranes

Correia, D.M.; Padrão, J; Rodrigues, L. R.; Dourado, F.; Lanceros-Mendez, S.; Sencadas, V.

The thermal and hydrolytic degradation of electrospun gelatin membranes cross-linked with glutaraldehyde in vapor phase has been studied. In vitro degradation of gelatin membranes was evaluated in phosphate buffer saline solution at 37 ºC. After 15 days under these conditions, a weight loss of 68 % was observed, attributed to solvation and depolymerization of the main polymeric chains. Thermal degradation kinet...

Data: 2013   |   Origem: CiencIPCA

Fluorescence in situ hybridization method using a peptide nucleic acid probe fo...

Machado, António; Almeida, Carina; Carvalho, Ana; Boyen, Filip; Haesebrouck, Freddy; Rodrigues, L. R.; Cerca, Nuno; Azevedo, N. F.

Lactobacillus species constitute one of the dominant and beneficial bacteria in our body and are used in developed countries as a microbial adjuvant. Identification of these probiotic bacteria is traditionally performed by culture-based techniques. However, such methods are very time-consuming and can give inaccurate results, especially when Lactobacillus is present in mixed bacterial complex communities. Our s...


Fluorescence in situ hybridization method using peptide nucleic acid probes for...

Machado, António; Almeida, Carina; Salgueiro, D. A. L.; Henriques, Ana Filipa; Vaneechoutte, Mario; Haesebrouck, Freddy; Vieira, M. J.; Rodrigues, L. R.

Background: Bacterial vaginosis (BV) is a common vaginal infection occurring in women of reproductive age. It is widely accepted that the microbial switch from normal microflora to BV is characterized by a decrease in vaginal colonization by Lactobacillus species together with an increase of Gardnerella vaginalis and other anaerobes. Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluore...


New trends in biotechnological processes to increase the environmental protection

Moldes, Ana; Domínguez González, José Manuel; Rodrigues, L. R.; Converti, Attilio


Milk minor constituents, enzymes, hormones, growth factors, and organic acids

Rodrigues, L. R.

Milk and derived products contain essential nutrients, such as proteins, lactose, minerals, vitamins, and enzymes. Additionally, despite of their low concentrations in milk, many other minor constituents present important physiological and/or technological roles (e.g. hormones, growth factors). Dairy industries face many challenges regarding milk processing. Also, the full knowledge on these constituents’ physi...


Optimization and characterization of biosurfactant production by Bacillus subti...

Pereira, J. F.; Gudiña, Eduardo J.; Costa, Rita; Vitorino, Rui; Teixeira, J. A.; Coutinho, João A. P.; Rodrigues, L. R.

Biosurfactant production by three Bacillus subtilis strains (#309, #311 and #573) isolated from Brazilian crude oils was optimized based on different carbon and nitrogen sources. The lowest surface tension values were obtained using sucrose containing media for the three isolates. Biosurfactants produced by each strain were characterized by Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic...


Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate...

Gonçalves, S.; Leirós, Ana Catarina Correia; Van Kooten, Theo; Dourado, Fernando; Rodrigues, L. R.

Poly(dimethyl siloxane) (PDMS) was surface-polymerized with poly(ethylene glycol)methacrylate (PEGMA) by surface-initiated atom transfer radical polymerization (SI-ATRP) in aqueous media at room temperature. Modification of the PDMS surface followed a three-step procedure: (i) PDMS surface hydroxylation by UV/ozone exposure, immediately followed by (ii) covalent attachment of the initiator, 1-trichlorosilyl-2-(...


Partial characterization of biosurfactant from Lactobacillus pentosus and compa...

Moldes, Ana; Paradelo, R.; Vecino, X.; Cruz, J. M.; Gudiña, Eduardo J.; Rodrigues, L. R.; Teixeira, J. A.; Dominguez, José Manuel; Barral, M. T.

The capability of a cell bound biosurfactant produced by Lactobacillus pentosus, to accelerate the bioremediation of a hydrocarbon-contaminated soil, was compared with a synthetic anionic surfactant (sodium dodecyl sulphate SDS-). The biosurfactant produced by the bacteria was analyzed by Fourier transform infrared spectroscopy (FTIR) that clearly indicates the presence of OH and NH groups, C=O stretching of ca...


Antimicrobial and anti-adhesive potential of biosurfactants produced by Candida...

Rufino, R. D.; Luna, J. M.; Sarubbo, L. A.; Rodrigues, L. R.; Teixeira, J. A.; Takaki, G. M. Campos


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia