Multiple transcripts regulate glucose-triggered mRNA decay of the lactate trans...

• Autor(es): Andrade, Raquel P. ; Kötter, Peter ; Entian, Karl-Dieter ; Casal, Margarida
• Data: 2005
• Identificador Persistente: http://hdl.handle.net/1822/2915
• Origem: RepositóriUM - Universidade do Minho
• Assunto(s): Yeast; mRNA degradation; Glucose repression; JEN1
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

The Saccharomyces cerevisiae JEN1 gene encoding the lactate transporter undergoes strong catabolic repression at both transcriptional and post-transcriptional levels. JEN1 mRNA decay is greatly accelerated upon the addition of a pulse of glucose, fructose or mannose to induced cell cultures. Mapping of the 5´UTRs and 3´UTRs of JEN1 transcripts revealed multiple transcription start-sites located at position -51, +391 or +972, depending on the cell culture conditions. The presence of the JEN1(+391) transcript correlated with rapid glucose-triggered mRNA degradation of the JEN1(-51) transcript, whereas when the small transcript started at position +972, the JEN1(-51) mRNA turnover rate was unaffected. Overexpressed JEN1(+391) transcript accelerated JEN1(-51) mRNA decay in all conditions tested but was not translated. We propose that the JEN1(+391) transcript may have a ‘‘sensor-like’’ function, regulating glucose-triggered degradation of JEN1(-51) protein-coding mRNA.