Encontrados 11 documentos, a visualizar página 1 de 2
Timing embryo segmentation : dynamics and regulatory mechanisms of the vertebra...
Resende, Tatiana; Andrade, Raquel P.; Palmeirim, I.All vertebrate species present a segmented body, easily observed in the vertebrate column and its associated components, which provides a high degree of motility to the adult body and efficient protection of the internal organs. The sequential formation of the segmented precursors of the vertebral column during embryonic development, the somites, is governed by an oscillating genetic network, the somitogenesis ...
The impact of alternative science education methodologies on the motivation and...
Azevedo, Maria Manuel; Fonseca, Fernando; Andrade, Raquel P.; Palmeirim, I.Fully aware of the increasing importance of active and experimental learning, this study has been conducted in Portugal between 2007 and 2008 and was the first of this kind to ever be conducted in this country. Thirty eight students of the school E.B. 2, 3 D. Maria II (middle school) participated in the extra-curricular activity entitled “Scientists for a Day”, previous developed by the Life and Health Science ...
Joint interpretation of AER/FGF and ZPA/SHH over time and space underlies hairy...
Sheeba, Caroline J.; Palmeirim, I.; Andrade, Raquel P.The authors are grateful to Cheryll Tickle and Lewis Wolpert for helpful comments and critical reading of the manuscript, to Lisa Gonc¸alves and Tatiana Resende for fruitful discussions, Rute Moura and Paulina Piairo for technical assistance and Baolin Wang for the kind gift of Gli3 antibody. ; Embryo development requires precise orchestration of cell proliferation and differentiation in both time and space. A...
The impact of experimental activities on the motivation and acquisition of scie...
Azevedo, Maria Manuel; Fonseca, Fernando; Brito, C.; Andrade, Raquel P.; Palmeirim, I.; Teixeira, Alexandra Sofia PereiraForty students (middle school) participated in the science activity entitled “Scientists for a Day”, developed by the Life and Health Science Research Institute/School of Health Science, University of Minho. The activity consisted in a set of laboratory activities. The general objectives of this activity were to stimulate the pleasure of knowledge, encourage critical views, heighten the interest in science, mot...
Sonic hedgehog in temporal control of somite formation
Resende, Tatiana; Ferreira, M??nica; Teillet, Marie-Aim??e; Tavares, Ana Teresa; Andrade, Raquel P.; Palmeirim, I.Vertebrate embryo somite formation is temporally controlled by the cyclic expression of somitogenesis clock genes in the presomitic mesoderm (PSM). The somitogenesis clock is believed to be an intrinsic property of this tissue, operating independently of embryonic midline structures and the signaling molecules produced therein, namely Sonic hedgehog (Shh). This work revisits the notochord signaling contribution...
Multiple transcripts regulate glucose-triggered mRNA decay of the lactate trans...
Andrade, Raquel P.; Kötter, Peter; Entian, Karl-Dieter; Casal, MargaridaThe Saccharomyces cerevisiae JEN1 gene encoding the lactate transporter undergoes strong catabolic repression at both transcriptional and post-transcriptional levels. JEN1 mRNA decay is greatly accelerated upon the addition of a pulse of glucose, fructose or mannose to induced cell cultures. Mapping of the 5´UTRs and 3´UTRs of JEN1 transcripts revealed multiple transcription start-sites located at position -51,...
Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae...
Silva, Isabel Soares; Schuller, Dorit; Andrade, Raquel P.; Baltazar, Fátima; Cássio, Fernanda; Casal, MargaridaIn Saccharomyces cerevisiae the activity for the lactate proton-symporter in dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western-blotting analyses indicated the expression of JEN1 in the transformants. Lactate permease activity was obtained in P. pastoris cells with a Vmax of 2.1 nmol s-1 ...
Exploring genetic tools for the overexpression of the lactate permease Jen1p of...
Silva, Isabel Soares; Andrade, Raquel P.; Schuller, Dorit; Casal, MargaridaResumo e poster da comunicação apresentada sob forma de painel no congresso "XXI International Conference on Yeast Genetics and Molecular Biology", em 2003, em Göteborg, Suécia. ; In Saccharomyces cerevisiae the active transport of monocarboxylic acids is dependent upon the expression of the permease Jen1p. Cells of S. cerevisiae jen1 were transformed with the plasmids p416GPD (centromeric) and p426GPD (multic...
Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae...
Silva, Isabel Soares; Schuller, Dorit; Andrade, Raquel P.; Baltazar, Fátima; Cássio, Fernanda; Casal, MargaridaPichia pastoris was transformed with an integrative plasmid containing the Saccharomyces cerevisiae JEN1 gene. After 24 h of methanol induction, Northern and Western-blotting analyses indicated the expression of JEN1 in the transformants. Lactate permease activity was obtained in P. pastoris cells with a Vmax of 2.1 nmol s-1 mg-1 dry weight. Reconstitution of the lactate permease activity was achieved by fusing...
Permease de monocarboxilatos de Saccharomyces cerevisiae (JEN1) : expressão con...
Schuller, Dorit; Andrade, Raquel P.; Silva, Isabel Soares; Casal, MargaridaResumo da comunicação oral apresentada no encontro científico "10as Jornadas de Biologia de Leveduras Professor Nicolau van Uden", em 2002, Faro, Portugal. ; Células da estirpe W303-1A jen1∆ foram transformadas com os plasmídeos pD1 e pD2, onde a ORF que codifica para o JEN1 foi clonada sob o controlo do promotor constitutivo GPD. O transporte de ácido láctico foi estimado em células transformadas com estes pl...
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