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Multiple transcripts regulate glucose-triggered mRNA decay of the lactate trans...
Andrade, Raquel P.; Kötter, Peter; Entian, Karl-Dieter; Casal, MargaridaThe Saccharomyces cerevisiae JEN1 gene encoding the lactate transporter undergoes strong catabolic repression at both transcriptional and post-transcriptional levels. JEN1 mRNA decay is greatly accelerated upon the addition of a pulse of glucose, fructose or mannose to induced cell cultures. Mapping of the 5´UTRs and 3´UTRs of JEN1 transcripts revealed multiple transcription start-sites located at position -51,...
The disruption of JEN1 from Candida albicans impairs the transport of lactate
Paiva, Sandra; Silva, Isabel Soares; Kötter, Peter; Entian, Karl-Dieter; Casal, MargaridaPoster apresentado em XXI International Conference on Yeast Genetics and Molecular Biology, Gotemburgo, Suécia, 7 Jul. - 12 Jul. 2004
The disruption of JEN1 from Candida albicans impairs the transport of lactate
Silva, Isabel Soares; Paiva, Sandra; Kötter, Peter; Entian, Karl-Dieter; Casal, MargaridaA lactate permease was biochemically identified in Candida albicans RM1000 presenting the following kinetic parameters at pH 5.0: Km 0.33 ± 0.09 mM and Vmax 0.85± 0.06 nmol s-1 mg dry wt-1. Lactate uptake was competitively inhibited by pyruvic and propionic acids; acetic acid behaved as a non-competitive substrate. An ORF homologous to Saccharomyces cerevisiae gene JEN1 was identified (CaJEN1). Deletions of bot...
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