Secondary structure of rhBMP-2 in a protective biopolymeric carrier material

• Author(s): Gilde, Flora ; Maniti, Ofélia ; Guillot, Raphael ; Mano, J. F. ; Logeart-Avramoglou, Delphine ; Sailhan, Frédéric ; Picart, Catherine
• Date: 2012
• Persistent ID: http://hdl.handle.net/1822/24905
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): ATR-FTIR; Differentiation; Growth-factors; Inhibition; Microenvironments; One morphogenetic protein-2; Polyelectrolyte multilayer films; Stability; Tissue; BMP-2
• Document Type: Article
• Language: English

Description

Efficient delivery of growth factors is one of the great challenges of tissue engineering. Polyelectrolyte multilayer films (PEM) made of biopolymers have recently emerged as an interesting carrier for delivering recombinant human bone morphogenetic protein 2 (rhBMP-2 noted here BMP-2) to cells in a matrix-bound manner. We recently showed that PEM made of poly(l-lysine) and hyaluronan (PLL/HA) can retain high and tunable quantities of BMP-2 and can deliver it to cells to induce their differentiation in osteoblasts. Here, we investigate quantitatively by Fourier transform infrared spectroscopy (FTIR) the secondary structure of BMP-2 in solution as well as trapped in a biopolymeric thin film. We reveal that the major structural elements of BMP-2 in solution are intramolecular β-sheets and unordered structures as well as α-helices. Furthermore, we studied the secondary structure of rhBMP-2 trapped in hydrated films and in dry films since drying is an important step for future applications of these bioactive films onto orthopedic biomaterials. We demonstrate that the structural elements were preserved when BMP-2 was trapped in the biopolymeric film in hydrated conditions and, to a lesser extent, in dry state. Importantly, its bioactivity was maintained after drying of the film. Our results appear highly promising for future applications of these films as coatings of biomedical materials, to deliver bioactive proteins while preserving their bioactivity upon storage in dry state.