Descrição
Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010 Salmonella and Campylobacter are recognized worldwide as the major foodborne pathogens
responsible for human gastrointestinal diseases. The increased resistance of bacteria to
antibiotics has encouraged the development of alternatives to control bacterial pathogens.
Bacteriophages (phages), as natural predators of bacteria, are considered an appealing
option. We report herein the isolation and genome characterisation of two Myoviridae broad
lytic spectra Campylobacter (vB_CcoM-IBB35) and Salmonella phages (PVP-SE1) with high
potential for therapy. The majority of genes of vB_CcoM-IBB35 are unique although
homology exists with members of the Teequatrovirinae. Unique genes involved in
pathogenesis, carbohydrate and amino acid metabolism were also observed along with
several incidences of gene duplications, split genes with intein and introns and “insertion-like
sequences”.
From the 244 genes found in PVP-SE1, approximately 46% encode unique proteins and only
22.1% exhibited homology with known proteins. The genome sequence presents high
homology (145 gene encoding proteins) with the E.coli bacteriophage rV5, both unrelated to
any other known phage, which might suggest that they belong to a new phage genetic group.
As conclusion, one can argue that the genomic characterization of both phages did not
reveal any factor which could preclude its therapeutic use.
; Carvalho, Carla A. O. C. M. 
