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A suggested classification for two groups of Campylobacter myoviruses

Javed, Muhammad Afzal; Ackermann, H. W.; Azeredo, Joana; Carvalho, Carla M.; Connerton, Ian; Evoy, Stephane; Hammerl, Jens Andre; Hertwig, Stefan

Most Campylobacter bacteriophages isolated to date have long contractile tails and belong to the family Myoviridae. Based on their morphology, genome size and endonuclease restriction profile, Campylobacter phages were originally divided into three groups. The recent genome sequencing of seven virulent campylophages reveal further details of the relationships between these phages at the genome organization leve...


Complete genome sequence of the broad-host-range Paenibacillus larvae phage phi...

Oliveira, Ana; Melo, Luís D. R.; Kropinski, Andrew M.; Azeredo, Joana

Paenibacillus larvae is a Gram-positive bacterium that causes American foulbrood, an important disease in apiculture. We report the first complete genome sequence of a P. larvae phage, phiIBB_Pl23, isolated from a hive in northern Portugal. This phage belongs to the family Siphoviridae.


Genomic characterization of the Staphylococcus epidermidis-specific bacteriopha...

Melo, Luís D. R.; Oliveira, F.; Sillankorva, Sanna; Pérez-Cabezas, B.; Ackermann, H. W.; Kropinski, Andrew M.; Vilanova, Manuel; Azeredo, Joana

Staphylococcus epidermidis is one of the most frequent causative agents of nosocomial infections, predominantly in patients with indwelling medical devices. This microrganism may form biofilms which are microbial structures very tolerant to the host immune defenses and to antibiotherapy. Therefore, studies are needed in order to develop effective methods for biofilm control. Currently, bacteriophages (phages) a...


Genome sequence of the broad-host-range Pseudomonas phage Phi-S1

Sillankorva, Sanna; Kropinski, Andrew M.; Azeredo, Joana

The broad-host-range lytic Pseudomonas phage Φ-S1 possess a 40,192 bp double-stranded DNA (dsDNA) genome of 47 open reading frames (ORFs) and belongs to the family Podoviridae, subfamily Autographivirinae, genus T7likevirus.


Complete genome sequence of the lytic Pseudomonas fluorescens phage ϕIBB-PF7A

Sillankorva, Sanna; Kluskens, Leon; Linghorn, E.; Kropinski, Andrew M.; Neubauer, P.; Azeredo, Joana

Background Phage ϕIBB-PF7A is a T7-like bacteriophage capable of infecting several Pseudomonas fluorescens dairy isolates and is extremely efficient in lysing this bacterium even when growing in biofilms attached to surfaces. This work describes the complete genome sequence of this phage. Results The genome consists of a linear double-stranded DNA of 40,973 bp, with 985 bp long direct terminal repeats and a G...


The genome and proteome of a Campylobacter coli bacteriophage vB_CcoM-IBB_35 re...

Carvalho, Carla M.; Kropinski, Andrew M.; Lingohr, Erika J.; Santos, Sílvio; King, Jonathan; Azeredo, Joana

Campylobacter is the leading cause of foodborne diseases worldwide. Bacteriophages (phages) are naturally occurring predators of bacteria, ubiquitous in the environment, with high host specificity and thus considered an appealing option to control bacterial pathogens. Nevertheless for an effective use of phages as antimicrobial agents, it is important to understand phage biology which renders crucial the analys...


Phages as therapeutic tools to control major foodborne pathogens : campylobacte...

Carvalho, Carla M.; Santos, Sílvio; Kropinski, Andrew M.; Ferreira, E. C.; Azeredo, Joana


Genomic and proteomic characterization of the broad host range Salmonella phage...

Santos, Sílvio; Kropinski, Andrew M.; Ceyssens, P. J.; Ackermann, H. W.; Villegas, A.; Carvalho, Carla M.; Lavigne, Rob; Krylov, V. N.; Ferreira, E. C.

(Bacterio)phage PVP-SE1, isolated from a German wastewater plant, presents a high potential value as a biocontrol agent and as a diagnostic tool, even compared to the well-studied typing phage Felix 01, due to its broad lytic spectrum against different Salmonella strains. Sequence analysis of its genome (145,964 bp) shows it to be terminally redundant and circularly permuted. Its G C content, 45.6 mol%, is lowe...


Genome sequence of the broad host range salmonella bacteriophage phi PVP-SE1

Santos, Sílvio; Kropinski, Andrew M.; Villegas, André; Krylov, Victor; Carvalho, Carla M.; Ferreira, E. C.; Azeredo, Joana

Salmonella is an important zoonotic pathogen and remains the primary cause of reported food poisoning worldwide with massive outbreaks. The increased resistance of Salmonella to antibiotics and other biocides has encouraged the development of alternatives to chemotherapy such as phage therapy. Phage phi PVP-SE1 is a salmonella phage isolated from a Germany (Regensburg) wastewater plant. This phage is characteri...


Genome characterization of two valuable therapeutic bacteriophages against Salm...

Santos, Sílvio; Carvalho, Carla A. O. C. M.; Kropinski, Andrew M.; Ferreira, E. C.; Azeredo, Joana

Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010 ; Salmonella and Campylobacter are recognized worldwide as the major foodborne pathogens responsible for human gastrointestinal diseases. The increased resistance of bacteria to antibiotics has encouraged the development of alternatives to control bacterial pathogens. Bacteriophages (phages), as nat...


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