Gene knockdown in Paracoccidioides brasiliensis using antisense RNA

• Autor(es): Menino, João F. ; Almeida, A. J. ; Rodrigues, Fernando José dos Santos
• Data: 2012
• Identificador Persistente: http://hdl.handle.net/1822/22773
• Origem: RepositóriUM - Universidade do Minho
• Assunto(s): Paracoccidioides brasiliensis; Molecular techniques; aRNA technology; Gene knockdown
• Tipo de Documento: Parte ou capítulo de livro
• Idioma: Inglês

Descrição

Paracoccidioides brasiliensis is a thermal dimorphic fungus which at host environment exhibits a multinucleated and multibudding yeast form. The cellular and molecular mechanisms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in P. brasiliensis by antisense RNA (aRNA) technology taking advantage of an Agrobacterium tumefaciens-mediated transformation (ATMT) system. Together, these techniques represent a reliable toolbox that can be employed for functional genetic analysis of putative virulence factors and morphogenic regulators, aiming to the identification of new potential drug targets.