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Genetic PTX3 deficiency and aspergillosis in stem-cell transplantation
Rodrigues, Fernando José dos Santos; Cunha, Cristina; Aversa, Franco; Lacerda, João F.; Busca, Alessandro; Kunzai, Oliver; Grube, MatthiasBACKGROUND: The soluble pattern-recognition receptor known as long pentraxin 3 (PTX3) has a nonredundant role in antifungal immunity. The contribution of single-nucleotide polymorphisms (SNPs) in PTX3 to the development of invasive aspergillosis is unknown. METHODS: We screened an initial cohort of 268 patients undergoing hematopoietic stem-cell transplantation (HSCT) and their donors for PTX3 SNPs modifying th...
Functional characterization of a xylose transporter in Aspergillus nidulans
Rodrigues, Fernando José dos Santos; Menino, João F.; Colabardini, Ana Cristina; Ries, Laure Nicolas Annick; Brown, Neil AndrewBACKGROUND: The production of bioethanol from lignocellulosic feedstocks will only become economically feasible when the majority of cellulosic and hemicellulosic biopolymers can be efficiently converted into bioethanol. The main component of cellulose is glucose, whereas hemicelluloses mainly consist of pentose sugars such as D-xylose and L-arabinose. The genomes of filamentous fungi such as A. nidulans encode...
Involvement of yeast HSP90 isoforms in response to stress and cell death induce...
Silva, Maria Alexandra; Marques, Belém Sampaio; Fernandes, Ângela Margarida Oliveira; Carreto, Laura; Rodrigues, Fernando José dos SantosAcetic acid-induced apoptosis in yeast is accompanied by an impairment of the general protein synthesis machinery, yet paradoxically also by the up-regulation of the two isoforms of the heat shock protein 90 (HSP90) chaperone family, Hsc82p and Hsp82p. Herein, we show that impairment of cap-dependent translation initiation induced by acetic acid is caused by the phosphorylation and inactivation of eIF2 alpha by...
Mycobacterium tuberculosis strains are differentially recognized by TLRs with a...
Carmona, J. A.; Cruz, Andrea; Teixeira, Lucia de Fatima Moreira; Sousa, Carole Lara Veiga de; Sousa, Jeremy; Osório, Nuno S.; Saraiva, Ana L.Tuberculosis associates with a wide spectrum of disease outcomes. The Beijing (Bj) lineage of Mycobacterium tuberculosis (Mtb) is suggested to be more virulent than other Mtb lineages and prone to elicit non-protective immune responses. However, highly heterogeneous immune responses were reported upon infection of innate immune cells with Bj strains or stimulation with their glycolipids. Using both in vitro and...
TLR9 activation dampens the early inflammatory response to paracoccidioides bra...
Menino, João F.; Saraiva, Margarida; Alves, Ana G. Gomes; Silva, Diogo; Sturme, Mark H. J.; Gomes, Jéssica Angélica Rezende; Pedrosa, JorgeBackground: Paracoccidioides brasiliensis causes paracoccidioidomycosis, one of the most prevalent systemic mycosis in Latin America. Thus, understanding the characteristics of the protective immune response to P. brasiliensis is of interest, as it may reveal targets for disease control. The initiation of the immune response relies on the activation of pattern recognition receptors, among which are TLRs. Both T...
The genome sequence of the highly acetic acid-tolerant zygosaccharomyces bailii...
Leão, Cecília; Rodrigues, Fernando José dos Santos; Santos, Júlia; Mira, Nuno P.; Münsterkötter, Martin; Valada, Filipa Dias; Palma, MargaridaIn this work, it is described the sequencing and annotation of the genome of the yeast strain ISA1307, isolated from a sparkling wine continuous production plant. This strain, formerly considered of the Zygosaccharomyces bailii species, has been used to study Z. bailii physiology, in particular, its extreme tolerance to acetic acid stress at low pH. The analysis of the genome sequence described in this work ind...
Identification of glucose transporters in Aspergillus nidulans
Reis, Thaila Fernanda dos; Menino, João F.; Pedro Bom, Vinícius Leite; Brown, Neil Andrew; Colabardini, Ana Cristina; Savoldi, Marcelao characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae HxtB-E were targeted ...
The rs5743836 polymorphism in TLR9 confers a population-based increased risk of...
Carvalho, Agostinho; Cunha, Cristina; Almeida, A. J.; Osório, Nuno S.; Saraiva, Margarida; Coelho, Maria Teixeira; Pedreiro, Susana; Torrado, EgídioWe are grateful to Paulo Vieira, Cecília Leão, Manuel T. Silva, Nuno Sousa, Jorge Correia- Pinto, Joana Palha, Margarida Correia-Neves, Margarida Lima and Matthew Berry for all their input throughout these studies and critical reading of the manuscript. We are grateful to the patients who joint this study as well as to all members of the Life and Health Sciences Research Institute and School of Health Sciences,...
Functionality of the paracoccidioides mating α-pheromone-receptor system
Gomes, Jéssica Angélica Rezende; Alves, Ana G. Gomes; Menino, João F.; Coelho, Marco; Ludovico, Paula; Gonçalves, Paula; Sturme, Mark H. J.Recent evidence suggests that Paracoccidioides species have the potential to undergo sexual reproduction, although no sexual cycle has been identified either in nature or under laboratory conditions. In the present work we detected low expression levels of the heterothallic MAT loci genes MAT1-1 and MAT1-2, the a-pheromone (PBa) gene, and the a- and apheromone receptor (PREB and PREA) genes in yeast and mycelia...
Gene knockdown in Paracoccidioides brasiliensis using antisense RNA
Menino, João F.; Almeida, A. J.; Rodrigues, Fernando José dos SantosParacoccidioides brasiliensis is a thermal dimorphic fungus which at host environment exhibits a multinucleated and multibudding yeast form. The cellular and molecular mechanisms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in P. brasiliensis by antisense RNA (aRNA) t...
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