Fluorescence probe techniques to monitor protein adsorption-induced conformatio...

• Author(s): Benesch, Johan ; Hungerford, Graham ; Suhling, Klaus ; Tregidgo, Carolyn ; Mano, J. F. ; Reis, R. L.
• Date: 2007
• Persistent ID: http://hdl.handle.net/1822/13738
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Fluorescence; Anisotropy; Time resolved; Albumin; Protein conformation; Fluorescence lifetime imaging; Synchronous scan; Tryptophan; Nile red; Fluorescein isothiocyanate
• Document Type: Article
• Language: English

Description

The study of protein adsorption and any associated conformational changes on interaction with biomaterials is of great importance in the area of implants and tissue constructs. This study aimed to evaluate some fluorescent techniques to probe protein conformation on a selection of biodegradable polymers currently under investigation for biomedical applications. Because of the fluorescence emanating from the polymers, the use of monitoring intrinsic protein fluorescence was precluded. A highly solvatochromic fluorescent dye, Nile red, and a well-known protein label, fluorescein isothiocyanate, were employed to study the adsorption of serum albumin to polycaprolactone and to some extent also to two starch-containing polymer blends (SPCL and SEVA-C). A variety of fluorescence techniques, steady state, time resolved, and imaging were employed. Nile red was found to leach from the protein, while fluorescein isothiocyanate proved useful in elucidating a conformational change in the protein and the observation of protein aggregates adsorbed to the polymer surface. These effects were seen by making use of the phenomenon of energy migration between the fluorescent tags to monitor interprobe distance and the use of fluorescence lifetime imaging to ascertain the surface packing of the protein on polymer.