Encontrados 11 documentos, a visualizar página 1 de 2
Hybrid biodegradable membranes of silane-treated chitosan/soy protein for biome...
Silva, S. S.; Oliveira, Joaquim M.; Benesch, Johan; Caridade, S. G.; Mano, J. F.; Reis, R. L.In recent years, progress in the field of hybrid materials has been accelerated through use of the sol–gel process for creating materials and devices, which benefit from the incorporation of both inorganic and organic components. In this work, organic–inorganic hybrid membranes were prepared from tetraethoxysilane and a blend system composed of chitosan and soy protein. By introducing a small amount of siloxane...
Analysing protein competition on self-assembled mono-layers studied with quartz...
Benesch, Johan; Mano, J. F.; Reis, R. L.The mechanisms by which proteins adsorb to surfaces of biomaterials have long been of interest. The present work started with the premise that small/hard and large/soft proteins will yield different sets of normalized frequency shift and dissipation signals when studied with a quartz crystal microbalance. The aim was to evaluate the usefulness of these raw data to study protein competition using protein incubat...
Plasma surface modification of chitosan membranes : characterization and prelim...
Silva, S. S.; Luna, Sandra M.; Gomes, Manuela E.; Benesch, Johan; Pashkuleva, I.; Mano, J. F.; Reis, R. L.Surface modification of biomaterials is a way to tailor cell responses whilst retaining the bulk properties. In this work, chitosan membranes were prepared by solvent casting and treated with nitrogen or argon plasma at 20Wfor 10–40 min. AFM indicated an increase in the surface roughness as a result of the ongoing etching process. XPS and contact angle measurements showed different surface elemental composition...
Proteins and their peptide motifs in acellular apatite mineralization of scaffo...
Benesch, Johan; Mano, J. F.; Reis, R. L.Many proteins in the inorganic=organic matrix of bone induce or modulate or inhibit mineralization of apatite in vivo. Many attempts have been made to mimic and understand this mechanism as part of bone formation, and ectopic mineralization and control thereof. Many attempts have also been made to use such proteins or protein fragments to harness their potential for improved mineralization. Such proteins and pe...
Surface engineered carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticl...
Oliveira, Joaquim M.; Kotobuki, Noriko; Marques, A. P.; Pirraco, Rogério; Benesch, Johan; Hirose, Motohiro; Costa, Silgia; Mano, J. F.; Hajime, OhgushiNovel highly branched biodegradable macromolecular systems have been developed by grafting carboxymethylchitosan (CMCht) onto low generation poly(amidoamine) (PAMAM) dendrimers. Such structures organize into sphere-like nanoparticles that are proposed to be used as carriers to deliver bioactive molecules aimed at controlling the behavior of stem cells, namely their proliferation and differentiation. The nanopar...
Fluorescence probe techniques to monitor protein adsorption-induced conformatio...
Benesch, Johan; Hungerford, Graham; Suhling, Klaus; Tregidgo, Carolyn; Mano, J. F.; Reis, R. L.The study of protein adsorption and any associated conformational changes on interaction with biomaterials is of great importance in the area of implants and tissue constructs. This study aimed to evaluate some fluorescent techniques to probe protein conformation on a selection of biodegradable polymers currently under investigation for biomedical applications. Because of the fluorescence emanating from the pol...
Morphology and miscibility of chitosan/soy protein blended membranes
Silva, S. S.; Goodfellow, Brian J.; Benesch, Johan; Rocha, J.; Mano, J. F.; Reis, R. L.A physico-chemical characterization of blended membranes composed by chitosan and soy protein has been carried out in order to probe the interactions that allow membranes to be formed from these biopolymer mixtures. These membranes are developed aiming at applications in wound healing and skin tissue engineering scaffolding. The structural features of chitosan/soy blended membranes were investigated by means of...
Effect of the labelling ratio on the photophysics of fluorescein isothiocyanate...
Hungerford, Graham; Benesch, Johan; Mano, J. F.; Reis, R. L.The non-linearity of the fluorescence emission on increasing the probe to protein ratio has long been regarded as problematic and has lead to the development of dyes to overcome this effect. One of the causes of this non-linear response can be ascribed to the overlap of the label’s own absorption and emission spectra. At higher labelling ratios, this affords the possibility of a reasonably efficient energy migr...
Blood protein adsorption onto chitosan
Benesch, Johan; Tengvall, P.Chitosan was recently indicated to enhance osteogenesis, improve wound healing but to activate the coagulation and the complement systems. In the present study approximately 10nm thick chitosan film were prepared on aminopropyltriethoxysilane (APTES) coated silicon. The surfaces were incubated in serum or plasma and subsequently in antibodies towards key complement and contact activation of coagulation proteins...
Protein adsorption to oligo (Ethylene Glycol) self assembled monolayers with am...
Benesch, Johan; Svedhem, S.; Svensson, S. C.; Valiokas, R.; Liedberg, B.; Tengvall, P.Low protein adsorption is believed advantageous for blood-contacting materials and ethylene glycols (EG)-based polymeric compounds are often attached to surfaces for this purpose. In the present study, the adsorption of brinogen, serum, and plasma were studied by ellipsometry on a series of well-de ned oligo(EG) terminated alkane-thiols self-assembled on gold. The layers were prepared with compounds of the g...
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