Maximization of fructose esters synthesis by response surface methodology

• Author(s): Neta, Nair S ; Peres, A. M. ; Teixeira, J. A. ; Rodrigues, L. R.
• Date: 2011
• Persistent ID: http://hdl.handle.net/1822/13063
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Candida antartica B; Fructose esters; Response surface methodology
• Document Type: Article
• Language: English

Description

Enzymatic synthesis of fructose fatty acide sterwasper formed inorganic solventmedia, using apurified lipase from Candida antartica B immobilizedinacrylicresin. Response sur face methodology with a central composit erotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature andre action time were found to bethe most significant parameters. The optimum operational conditions for maximizing the synthesis of fructos e esters were 57.18C, 100rpm and 37.8h. The model was validated in the identified optimal condition stocheck its adequacy and accuracy, and an experimental esterification percentage of 88.4% ( 0.3%) was obtained. These results showed that animprovement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions