The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglu...

• Author(s): Lemos, M. A. ; Teixeira, J. A. ; Domingues, M. R. M. ; Mota, M. ; Gama, F. M.
• Date: 2003
• Persistent ID: http://hdl.handle.net/1822/1086
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Cellulose binding domains; Synergism; Cellulases
• Document Type: Article
• Language: English

Description

The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.