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The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglu...
Lemos, M. A.; Teixeira, J. A.; Domingues, M. R. M.; Mota, M.; Gama, F. M.The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper...
Effect of Water Content on the Thermal Inactivation Kinetics of Horseradish Per...
Lemos, M. A.; Oliveira, J. C.; Saraiva, J. A.The thermal inactivation of horseradish peroxidase freeze-dried from solutions of different pH (8, 10 and 11.5, measured at 25 C) and equilibrated to different water contents was studied in the temperature range from 110 to 150 C. The water contents studied (0.0, 1.4, 16.2 and 25.6 g water per 100 g of dry enzyme) corresponded to water activities of 0.0, 0.11, 0.76 and 0.88 at 4 C. The kinetics were well descri...
Enzymatic upgrade of old paperboard containers
Pala, H.; Lemos, M. A.; Mota, M.; Gama, F. M.The use of enzymes in secondary fiber (old paperboard containers) upgrading was investigated. The following aspects were analyzed: (i) the effect of several enzymes and (non-hydrolytic) cellulose-binding domains on the pulp and paper properties; (ii) factors influencing enzymatic treatment of secondary fiber: enzyme dosage and reaction time; and (iii) enzyme action on fractions with different fiber-length. In g...
A simple method to separate cellulose-binding domains of fungal cellulases afte...
Lemos, M. A.; Teixeira, J. A.; Mota, M.; Gama, F. M.Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.
Variação fenotípica em acessos de açaizeiro (Euterpe oleracea Mart.) para carac...
OLIVEIRA, M. do S. P. de; LEMOS, M. A.; SANTOS, E. O. dos; SANTOS, V. F. dos.1998
Influence of the concentration of enzyme, phosphate ions and calcium chloride o...
Lemos, M. A.; Oliveira, J. C.; Saraiva, J. A.Resumo indisponível.
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