Detalhes do Documento

Influence of pH on cellular growth of Pichia pastoris KM71H by fed-batch process

Autor(es): Wanderley, Marcela Silvestre Outtes cv logo 1 ; Oliveira, Carla Cristina Marques de cv logo 2 ; Mussatto, Solange I. cv logo 3 ; Bruneska, Danyelly cv logo 4 ; Lima-Filho, José. L. cv logo 5 ; Domingues, Lucília cv logo 6 ; Teixeira, J. A. cv logo 7

Data: 2009

Identificador Persistente: http://hdl.handle.net/1822/10345

Origem: RepositóriUM - Universidade do Minho

Assunto(s): Pichia pastoris; Fed‐batch fermentation; Cellular growth; pH; Expression system


Descrição
Pichia pastoris is a methylotrophic yeast that can be genetically engineered to express proteins for industrial use. One of the most important advantages of protein expression in P. pastoris is its capability of growing on minimal medium and efficiently secreting heterologous proteins with low secretion levels of endogenous proteins. Operational variables such as pH, temperature, stirring rate, among others, usually affect the microorganism’s growth during the fermentation processes. Therefore, the present work aimed to evaluate the influence of pH on cellular growth of P. pastoris KM71H by fed‐batch process. The fermentation run was carried out in a 1.6 L (total volume) bioreactor, being performed in two phases: In the first stage (24 h), the yeast was batchcultured in BMGH medium; while in the second stage (72 h), it was cultivated by feed‐batch operation with a feeding medium containing 50% glycerol and 12ml/l of trace metal solution. During the overall process, which lasted after 96 h, the aeration and temperature conditions were fixed at 10 ml\L.h, 1.5 vvm and 30°C, respectively. Different pH values were evaluated: 5.0, 5.5 and 6.0. Cellular growth was determined by measuring the fermentation broth UVspectrophotometric absorbance at 600 nm, which was correlated to a calibration curve (dry weight ´ optical density). Glycerol consumption was detected by HPLC analysis. P. pastoris KM71H successfully grew in all the evaluated pH values; but the highest biomass production was observed at pH 5.0 (98.79 g/L). Although P. pastoris is reported as being a microorganism able to grow over a wide pH range (from 3 to 7); it was not observed high cell density of P. pastoris KM71H strain when cultivated at pHs 5.5 and 6.0. High cellular growth is especially important for proteins secretion, as the concentration of product in the medium is roughly proportional to the concentration of cells in culture. Finally, these results reveal the possibility of obtaining high cell density of P. pastoris KM71H by fed‐bach cultivation at pH 5.0, which can be a suitable condition for the yeast application in heterologous proteins production.
Tipo de Documento Documento de conferência
Idioma Inglês
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