Encontrados 22 documentos, a visualizar página 1 de 3
Influence of trace elements supplementation on the production of recombinant fr...
Wanderley, Marcela Silvestre Outtes; Oliveira, Carla Cristina Marques de; Bruneska, Danyelly; Domingues, Lucília; Lima-Filho, José. L.; Teixeira, J. A.Frutalin, a galactose-specific lectin used to detect specific tumour markers, is a protein with low expression level in breadfruit. In the present study, fed-batch fermentation in a stirred tank bioreactor was used as a strategy to enhance protein production by a recombinant Pichia pastoris KM71H. By using this process, the production of recombinant frutalin was 4-fold higher than the value obtained in shaker f...
Expression of heterologous proteins by Pichia pastoris under increased air pres...
Lopes, Marlene; Oliveira, Carla Cristina Marques de; Domingues, Lucília; Mota, M.; Belo, Belo, IsabelThe P. pastoris expression system has gained acceptance as an important host organism for the production of heterologous proteins. Its expression system presents the advantage of the organism can grow to the desired cell density on glycerol as the carbon source and then on methanol for high level heterologous production. However, the oxygen limitation generally has a detrimental effect on the expression of for...
The effect of the electric field on lag phase, β-galactosidase production and p...
Castro, I.; Oliveira, Carla Cristina Marques de; Domingues, Lucília; Teixeira, J. A.; Vicente, A. A.Ethanol and β-galactosidase production from cheese whey may significantly contribute to minimise environmental problems while producing value from lowcost raw materials. In this work, the recombinant Saccharomyces cerevisiae NCYC869-A3/pVK1.1 flocculent strain expressing the lacA gene (coding for β-galactosidase) of Aspergillus niger under ADHI promoter and terminator was used. This strain shows high ethanol an...
Cytotoxic effects of native and recombinant frutalin, a plant galactose-binding...
Oliveira, Carla Cristina Marques de; Nicolau, Ana; Teixeira, J. A.; Domingues, LucíliaFrutalin is the α-D-galactose-binding lectin isolated from breadfruit seeds. Frutalin was obtained from two different sources: native frutalin was purified from its natural origin, and recombinant frutalin was produced and purified from Pichia pastoris. This work aimed to study and compare the effect of native and recombinant frutalin on HeLa cervical cancer cells proliferation and apoptosis. Furthermore, the i...
Isolation and molecular cloning of γ-terpinene synthase gene from thymus caespi...
Oliveira, Carla Cristina Marques de; Lima, Ana Sofia; Domingues, Lucília; Trindade, HelenaPoster session from thematic symposium 8.
Recombinant microbial systems for improved β-galactosidase production and biote...
Oliveira, Carla Cristina Marques de; Guimarães, Pedro M. R.; Domingues, Lucíliaβ-Galactosidases (EC 3.2.1.23) constitute a large family of proteins that are known to catalyze both hydrolytic and transgalactosylation reactions. The hydrolytic activity has been applied in the food industry for decades for reducing the lactose content in milk, while the transgalactosylation activity has been used to synthesize galacto-oligosaccharides and galactose containing chemicals in recent years. The m...
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation
Domingues, Lucília; Guimarães, Pedro M. R.; Oliveira, Carla Cristina Marques deLactose is an interesting carbon source for the production of several bio-products by fermentation, primarily because it is the major component of cheese whey, the main by-product of dairy activities. However, the microorganism more widely used in industrial fermentation processes, the yeast Saccharomyces cerevisiae, does not have a lactose metabolisation system. Therefore, several metabolic engineering approac...
Expression and production of recombinant frutalin in different expression syste...
Oliveira, Carla Cristina Marques de; Teixeira, J. A.; Domingues, LucíliaEste resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968 ; Frutalin is the alpha-D-galactose-binding lectin expressed in breadfruit seeds (Artocarpus incisa). This lectin may be used in cancer diagnostics/therapeutics due to its potential abi...
cDNA cloning and functional expression of the α-d-galactose-binding lectin frut...
Oliveira, Carla Cristina Marques de; Costa, Sofia M.; Teixeira, J. A.; Domingues, LucíliacDNA clones encoding frutalin, the α-d-galactose-binding lectin expressed in breadfruit seeds (Artocarpus incisa), were isolated and sequenced. The deduced amino acid sequences indicated that frutalin may be encoded by a family of genes. The NCBI database searches revealed that the frutalin sequence is highly homologous with jacalin and mornigaG sequences. Frutalin cDNA was re-amplified and cloned into the comm...
A comparative study of recombinant and native frutalin binding to human prostat...
Oliveira, Carla Cristina Marques de; Teixeira, J. A.; Schmitt, Fernando C.; Domingues, LucíliaBackground Numerous studies indicate that cancer cells present an aberrant glycosylation pattern that can be detected by lectin histochemistry. Lectins have shown the ability to recognise these modifications in several carcinomas, namely in the prostate carcinoma, one of the most lethal diseases in man. Thus, the aim of this work was to investigate if the α-D-galactose-binding plant lectin frutalin is able to d...
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