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Chemical composition and antioxidant activity of sulphated polysaccharides extr...
Rodríguez-Jasso, Rosa María; Mussatto, Solange I.; Pastrana, Lorenzo; Aguilar, Cristóbal N.; Teixeira, J. A.Sulphated polysaccharides (SP) were extracted from Fucus vesiculosus seaweed by using two different hydrothermal processes: microwave-assisted extraction (MAE) and autohydrolysis (AH). The extraction yields, chemical composition, and antioxidant activity of the polysaccharides extracted were determined and compared. Although both processes afforded SP with similar yields (18.2 mass % and 16.5 mass %, for MAE an...
Extraction of sulfated polysaccharides by autohydrolysis of brown seaweed Fucus...
Rodríguez-Jasso, Rosa María; Mussatto, Solange I.; Pastrana, Lorenzo; Aguilar, Cristóbal N.; Teixeira, J. A.The extraction of sulfated polysaccharides (fucoidan) by autohydrolysis (AH) of brown seaweed Fucus vesiculosus was studied. Experimental assays were performed under different conditions of temperature (160 to 200°C) and reaction time (10 to 30 min) according to a 22 central composite design, and the conditions able to maximize the fucoidan yield were selected. The alga degradation and the total sugar yield in ...
Maximization of fructooligosaccharides and β-fructofuranosidase production by A...
Mussatto, Solange I.; Ballesteros, Lina F.; Martins, Silvia; Maltos, Dulce A .F.; Aguilar, Cristóbal N.; Teixeira, J. A.The conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus ...
Antibacterial activity of crude methanolic extract and fractions obtained from ...
Martins, Silvia; Amorim, Elba L. C.; Sobrinho, Tadeu J. S. Peixoto; Saraiva, Antonio M.; Pisciottano, Maria N. C.; Aguilar, Cristóbal N.The emergence of new infections and increase of bacteria drug-resistance rise up the urgent need for the development of new antibacterial agents from natural sources. This study was designed to evaluate the antibacterial activity of the crude methanolic extract (CME) and fractions [hexane (H), dichloromethane (DCM), ethyl acetate (EA) and ethanol (Et)] obtained from Larrea tridentata (Sessé & Moc. Ex DC.) Covil...
Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic i...
Landaeta, R.; Aroca, G.; Acevedo, F.; Teixeira, J. A.; Mussatto, Solange I.The ethanol production from lignocellulosic feedstocks is considered a promising strategy to increase global production of biofuels without impacting food supplies. However, some compounds released during the hydrolysis of lignocellulosic materials are toxic for the microbial metabolism, causing low ethanol yield and productivity during the fermentation. As an attempt to overcome this problem, the present study...
Laccase production by free and immobilized mycelia of Peniophora cinerea and Tr...
Silvério, Sara C.; Moreira, Sérgio; Milagres, Adriane M. F.; Macedo, Eugénia A.; Teixeira, J. A.; Mussatto, Solange I.The production of laccase by immobilized mycelia of Peniophora cinerea and Trametes versicolor was studied. In an initial stage, experimental assays were performed in Erlenmeyer flasks using free and immobilized mycelium, and the performance of the fungal strains to produce the enzyme was compared. Both fungi adhered into the support material (a synthetic fiber), growing not only on the surface but also in the ...
Malolactic fermentation of wines with immobilised lactic acid bacteria : Influe...
Genisheva, Z.; Mussatto, Solange I.; Oliveira, J. M.; Teixeira, J. A.Corn cobs, grape skins and grape stems were evaluated as support materials for immobilization of the lactic acid bacteria Oenococcus oeni. The support materials with immobilized cells were further used in malolactic fermentation (MLF) of white wine. Viability of using the immobilized supports was evaluated in consecutive batch fermentations under different conditions of temperature, ethanol and SO2. Additionall...
Influence of extraction solvents on the recovery of antioxidant phenolic compou...
Meneses, Nuno G.T.; Martins, Sílvia; Teixeira, J. A.; Mussatto, Solange I.This study evaluated the efficacy of different solvents (methanol, ethanol, acetone, hexane, ethyl acetate, water, methanol:water mixtures, ethanol:water mixtures, and acetone:water mixtures) for extracting antioxidant phenolic compounds from brewer’s spent grains (BSGs). The extracts were characterized regarding the contents of total phenols, flavonoids, proteins and reducing sugars. Antioxidant activity was d...
Decolorization of salt-alkaline effluent with industrial reactive dyes by lacca...
Moreira Neto, S. L.; Mussatto, Solange I.; Machado, K. M. G.; Milagres, Adriane M. F.The discharge of highly coloured synthetic dye effluents into rivers and lakes is harmful to the water bodies, and therefore, intensive researches have been focussed on the decolorization of wastewater by biological, physical or chemical treatments. In the present study, 12 basidiomycetes strains from the genus Pleurotus, Trametes, Lentinus, Peniophora, Pycnoporus, Rigidoporus, Hygrocybe and Psilocybe were eval...
Influence of trace elements supplementation on the production of recombinant fr...
Wanderley, Marcela Silvestre Outtes; Oliveira, Carla Cristina Marques de; Bruneska, Danyelly; Domingues, Lucília; Lima-Filho, José. L.; Teixeira, J. A.Frutalin, a galactose-specific lectin used to detect specific tumour markers, is a protein with low expression level in breadfruit. In the present study, fed-batch fermentation in a stirred tank bioreactor was used as a strategy to enhance protein production by a recombinant Pichia pastoris KM71H. By using this process, the production of recombinant frutalin was 4-fold higher than the value obtained in shaker f...
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