Conjugated linoleic acid reduces permeability and fluidity of adipose plasma me...

• Author(s): Martins, Ana Paula ; Lopes, Paula A. ; Martins, Susana V. ; Madeira, Ana ; Santos, Nuno C. ; Prates, José A. M. ; Moura, Teresa F. ; Soveral, Graça
• Date: 2010
• Persistent ID: http://hdl.handle.net/10451/8039
• Origin: Repositório da Universidade de Lisboa
• Subject(s): Conjugated linoleic acid; Adipose plasma membrane; Glycerol permeability; Water permeability; Membrane fluidity; Obese Zucker rats
• Document Type: Article
• Language: English

Description

NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. July 2010; 398 (2): 199-204. Conjugated linoleic acid (CLA) is a dietary fatty acid frequently used as a body fat reducing agent whose effects upon cell membranes and cellular function remain unknown. Obese Zucker rats were fed atherogenic diets containing saturated fats of vegetable or animal origin with or without 1% CLA, as a mixture of cis(c)9,trans(t)11 and t10,c12 isomers. Plasma membrane vesicles obtained from visceral adi- pose tissue were used to assess the effectiveness of dietary fat and CLA membrane incorporation and its outcome on fluidity and permeability to water and glycerol. A significant decrease in adipose membrane fluidity was correlated with the changes observed in permeability, which seem to be caused by the incor- poration of the t10,c12 CLA isomer into membrane phospholipids. These results indicate that CLA supple- mentation in obese Zucker rats fed saturated and cholesterol rich diets reduces the fluidity and permeability of adipose membranes, therefore not supporting CLA as a body fat reducing agent through membrane fluidification in obese fat consumers.