Effect of dietary conjugated linoleic acid isomers on water and glycerol permea...

• Author(s): Soveral, Graça ; Martins, Ana Paula ; Martins, Susana V. ; Lopes, Paula A. ; Alfaia, Cristina M. ; Prates, José A. M. ; Moura, Teresa F.
• Date: 2009
• Persistent ID: http://hdl.handle.net/10451/8038
• Origin: Repositório da Universidade de Lisboa
• Subject(s): Conjugated linoleic acid; Water permeability; Glycerol permeability; Kidney; Membrane vesicles; Aquaporin
• Document Type: Article
• Language: English

Description

NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. May 2009; 383(1): 108-112. Conjugated linoleic acid (CLA) refers to a group of positional and geometrical isomers of linoleic acid in which the double bonds are conjugated. Dietary CLA has been associated with various health benefits although details of its molecular mode of action remain elusive. The effect of CLA supplemented to palm oil-based diets in Wistar rats, as a mixture of both or isolated c9,t11 and t10,c12 isomers, was examined on water and glycerol membrane permeability of kidney proximal tubule. Although water permeability was unaltered, an increase in glycerol permeability was obtained for the group supplemented with CLA mixture, even though the activation energy for glycerol permeation remained high. This effect was correlated with an increased CLA isomeric membrane incorporation for the same dietary group. These results suggest that diet supplementation with CLA mixture, in contrast to its individual isomers, may enhance membrane fluidity subsequently raising kidney glycerol reabsorption.