Evaluation of mini-VIDAS rapid test for detection of Listeria monocytogenes fro...

• Author(s): Vaz-Velho, Manuela ; Duarte, Gabriela ; Gibbs, Paul
• Date: 2000
• Persistent ID: http://hdl.handle.net/10400.14/6809
• Origin: Veritati - Repositório Institucional da Universidade Católica Portuguesa
• Subject(s): Cold-smoked fish; Listeria monocytogenes; Rapid immunological-based methods
• Document Type: Article
• Language: English

Description

This study was conducted to evaluate the efficacy of the mini-VIDAS Listeria monocytogenes (LMO) system (BioMe´rieux Vitek, Inc., Missouri, USA) for detection of L. monocytogenes in environmental and fish samples from three Portuguese cold-smoking plants and from their fresh fish suppliers. Mini-VIDAS-LMO is a fully automated system that uses fluorescent ELFA (Enzyme Linked Fluorescent Assay) technology for detection of Listeria monocytogenes antigens in food. It can be a rapid screening method alternative to time consuming classical isolation and identification. Two hundred and ninety five samples were tested in mini-VIDAS-LMO and in parallel by the ISO 11290-1 traditional protocol. The mini-VIDAS-LMO detected 8 of the 11 confirmed positive samples and presented 11 false positive results. The specificity of the mini-VIDASLMO found in this experiment was 0.96 and the sensitivity 0.73