Milk from two different cow breeds, Barrosã and Frísia, was studied. Within the same breed five and four milk samples were taken respectively for chemical and microbiological analysis. The following chemical parameters were analysed in triplicate: Total Fat, Fatty Acids Identification and Quantification, Protein, Casein, Albumin, Non-protein Nitrogen, Vitamin A, Vitamin D, Phosphorus, Potassium, Sodium, Iron, M...
Low levels of occurrence of Listeria spp. in fresh salmon (Salmo_salar ) and salmon-trout (Oncorhynchus mykiss), may be related to the selectivity of the pre-enrichment broth recommended by ISO 11290-1. The purpose of this study was to compare the abilities of Fraser base (without supplements) and 0.1% (w/v) peptone water for recovering Listeria spp. from the fresh fish samples. Fifty-six fish were swabbed and...
Enhanced haemolysis agar (EHA) was compared to the two conventional Listeria isolation agars Oxford and PALCAM for its ability to detect Listeria spp. from production lines of fresh to cold-smoked fish. The ability of EHA for distinguishing L. monocytogenes colonies from other Listeria spp. was also evaluated. A total of 243 fish and environmental samples were analysed. Overall, 42 samples were found to contai...
The suitability of Listeria innocua 2030c, a tetracycline-resistant strain, to be used as an indicator for replacing Listeria monocytogenes in challenge studies with cold-smoked fish was ascertained. L. innocua 2030c was compared to serovars 4b and 1/2c of L. monocytogenes, the major types isolated from Portuguese cold-smoked fish products. Growth curves at 30°C, growth/survival patterns at 30°C under exposure ...
This study was conducted to evaluate the efficacy of the mini-VIDAS Listeria monocytogenes (LMO) system (BioMe´rieux Vitek, Inc., Missouri, USA) for detection of L. monocytogenes in environmental and fish samples from three Portuguese cold-smoking plants and from their fresh fish suppliers. Mini-VIDAS-LMO is a fully automated system that uses fluorescent ELFA (Enzyme Linked Fluorescent Assay) technology for det...
Four secondary enrichment protocols (conventional methods: UVM II, Fraser 24 h and Fraser 48 h: Impedimetric method: Listeria electrical detection medium) were studied for their ability to isolate Listeria spp. and Listeria monocytogenes from fish and environmental samples collected along the processing chain of cold-smoked fish. From all methods, Listeria spp. and L. monocytogenes were respectively present in ...
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