Effects of addition of sucrose and salt, and of starvation upon thermotolerance...

• Author(s): Carvalho, A. S. ; Silva, J. ; Ho, P. ; Teixeira, P. ; Malcata, F. X. ; Gibbs, P.
• Date: 2003
• Persistent ID: http://hdl.handle.net/10400.14/6692
• Origin: Veritati - Repositório Institucional da Universidade Católica Portuguesa
• Subject(s): Starter culture; Preservation; Growth medium; Viability
• Document Type: Article
• Language: English

Description

Increased survival of freeze-dried cells of Lactobacillus bulgaricus was observed when the drying medium was supplemented with sucrose; however, the magnitude of such protection was dependent on the growth medium used. Supplementing the growth medium with NaCl markedly increased survival of dried cells, and only a small effect was exerted by the composition of the drying medium or prior to starvation of cells. The D57 values of Lactobacillus bulgaricus cells grown in MRS were about half of those of cells grown in MRS supplemented with sucrose, with sucrose plus NaCl, or with NaCl.