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Expression and purification of the non-tagged LipL32 of pathogenic Leptospira

Hauk,P.; Carvalho,E.; Ho,P.L.

Leptospirosis is a reemerging infectious disease and the most disseminated zoonosis worldwide. A leptospiral surface protein, LipL32, only occurs in pathogenic Leptospira, and is the most abundant protein on the bacterial surface, being described as an important factor in host immunogenic response and also in bacterial infection. We describe here an alternative and simple purification protocol for non-tagged re...

Data: 2011   |   Origem: OASIS br

Trends in adjuvant development for vaccines: DAMPs and PAMPs as potential new a...

Miyaji,E.N.; Carvalho,E.; Oliveira,M.L.S.; Raw,I.; Ho,P.L.

Aluminum salts have been widely used in vaccine formulations and, after their introduction more than 80 years ago, only few vaccine formulations using new adjuvants were developed in the last two decades. Recent advances in the understanding of how innate mechanisms influence the adaptive immunity opened up the possibility for the development of new adjuvants in a more rational design. The purpose of this revie...

Data: 2011   |   Origem: OASIS br

Variability in quality of white and green beans during in-pack sterilization

Ávila, I.M.L.B.; Martins, R.C.; Ho, P.; Hendrickx, M.; Silva, C.L.M.

Non-uniformity in process quality was investigated during in-pack thermal sterilization of food products. This was accomplished through the combined application of the Monte Carlo procedure and a reliable mathematical method for process evaluation. Despite the large coefficients of variation found, the optimum quality process could be designed. The influence of the statistical variability of heating rate index ...


Genome features of Leptospira interrogans serovar Copenhageni

Nascimento,A.L.T.O.; Verjovski-Almeida,S.; Van Sluys,M.A.; Monteiro-Vitorello,C.B.; Camargo,L.E.A.; Digiampietri,L.A.; Harstkeerl,R.A.; Ho,P.L.

We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in mammals. Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a thr...

Data: 2004   |   Origem: OASIS br

A high-copy T7 Escherichia coli expression vector for the production of recombi...

Ramos,C.R.R.; Abreu,P.A.E.; Nascimento,A.L.T.O.; Ho,P.L.

We report here the construction of a vector derived from pET3-His and pRSET plasmids for the expression and purification of recombinant proteins in Escherichia coli based on T7 phage RNA polymerase. The resulting pAE plasmid combined the advantages of both vectors: small size (pRSET), expression of a short 6XHis tag at N-terminus (pET3-His) and a high copy number of plasmid (pRSET). The small size of the vector...

Data: 2004   |   Origem: OASIS br

Effect of various growth media upon survival during storage of freeze-dried Ent...

Carvalho, A.S.; Silva, J.; Ho, P.; Teixeira, P.; Malcata, F. X.; Gibbs, P.

The effects of three different growth media (MRS, M17 and Lee’s) on survival during freeze-drying and subsequent storage of six strains of Enterococcus faecalis and two strains of E. durans were investigated. Methods and Results: Distinct Enterococcus spp. strains were grown on M17, MRS and Lee’s broth, freeze-dried and stored at 20 C in air under darkness. At regular intervals throughout storage, freeze-dried ...


Effects of addition of sucrose and salt, and of starvation upon thermotolerance...

Carvalho, A. S.; Silva, J.; Ho, P.; Teixeira, P.; Malcata, F. X.; Gibbs, P.

Increased survival of freeze-dried cells of Lactobacillus bulgaricus was observed when the drying medium was supplemented with sucrose; however, the magnitude of such protection was dependent on the growth medium used. Supplementing the growth medium with NaCl markedly increased survival of dried cells, and only a small effect was exerted by the composition of the drying medium or prior to starvation of cells. ...


Multiple imputation and maximum likelihood principal component analysis of inco...

Ho, P.; Silva, M. C. M.; Hogg, T. A.

A multivariate data matrix containing a number of missing values was obtained from a study on the changes in colour and phenolic composition during the ageing of port. Two approaches were taken in the analysis of the data. The first involved the use of multiple imputation (MI) followed by principal components analysis (PCA). The second examined the use of maximum likelihood principal component analysis (MLPCA)....


Application of a liquid chromatographic method for the determination of phenoli...

Ho, P.; Hogg, T.A.; Silva, M.C.M.

Fortified wines which suffer extended periods of wood ageing develop characteristics which in many cases define the product. An important component of this style is contributed by the specific phenolic compounds and furans which are either extracted from the wood or formed during the barrel ageing process. An HPLC method is presented here for the determination of phenolic compounds and furans in wood aged forti...


CLONING AND EXPRESSION OF A POTENTIAL PRESYNAPTIC NEUROTOXIN PHOSPHOLIPASE A2-T...

Assni,A.; Carmona,E.; Campos,A.C.M.R.; Raw,I; Ho,P.L.

Data: 1997   |   Origem: OASIS br

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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia