Author(s):
Garrido, P. 
; Costa, Elísio ; Teixeira-Lemos, E. ; Parada, B. ; Teixeira, M. ; Santos, P. ; Piloto, N. ; Sereno, J. ; Alves, R. ; Pinto, R. ; Rocha-Pereira, P. ; Figueiredo, A. ; Nunes, S. ; Romão, A. M. ; Carvalho, L. ; Couceiro, P. ; Belo, L. ; Santos-Silva, A. ; Teixeira, F. ; Reis, F.
Date: 2010
Persistent ID: http://hdl.handle.net/10400.14/4428
Origin: Veritati - Repositório Institucional da Universidade Católica Portuguesa
Subject(s): Moderate chronic kidney disease; Partial nephrectomy; Rat model; Hemorheological data,; Renal function; Iron metabolism; CardioCardiovascular profile
Description
Chronic kidney disease (CKD) is
a major public health problem
throughout the world. The major outcomes
include a rapid progression,
with development of anaemia and
serious complications, namely thromboembol
ic and cardiovascular events.
The pathophysiological alterations
depend on the CKD degree, which
will also determine the moment to
initiate hemodialysis and recombinant
erythropoietin (rhEPO) therapies Thus, the cardio-renal complication
might be better prevented or
delayed if CKD patients are earlier
identified and treated for the associated
anaemia, which will depend on a
better characterization of moderate
stages of CKD. This study aimed to
characterization an animal of model
of moderate CKD induced by partial
(%) nephrectomy, by evaluating
hemorheological, biochemical and
cardiovascular profiles. Blood samples
from control and CKD rats were
collected at 0, 3, 9 and 15 weeks in order to evaluate: renal function,
hemorheological parameters, iron
metabolism, blood lipids, peripheral
sympathetic and serotonergic systems,
redox state and inflammatory
markers. BP, tissues uophism indexes
and kidney histomorphology
were also assessed. Our data is consistent
with a sustained moderate degree
of CKD with a quickly compensated
modest anaemia, though
presenting iron metabolism disturbances.
Despite the reasonable degree
of functionality of the remnant
kidney, as suggested by the anaemia
correction and by the kidney hypertrophy,
several important cardiovascular
modifications were developed.
Our model presented hypertension,
dyslipidaemia, erythropoietic disturbances,
sympathetic activation and
oxidative stress. This model might be
a good tool to study the cellular/molecularmechanisms underlying moderate
stages of CKD and to evaluate
the therapeutics efficacy for prevention,
treatment/correction of cardiorenal
anaemia syndromes and complications
in early stages.
