Treatment of cork boiling wastewater using chemical oxidation and biodegradation

• Author(s): Machado, Manuela Dias ; Madeira, Luis M. ; Nogales, Balbina ; Nunes, Olga C. ; Manaia, Célia M.
• Date: 2006
• Persistent ID: http://hdl.handle.net/10400.14/3364
• Origin: Veritati - Repositório Institucional da Universidade Católica Portuguesa
• Subject(s): Polyphenols; Mesophiles; Thermophiles; Tannic acid biodegradation; Fenton oxidation
• Document Type: Article
• Language: English

Description

Three cultures were enriched from cork boiling wastewater using tannic acid as the selective carbon substrate, at 25 C and pH 7.2, 25 C and pH 4.7 and 50 C and pH 4.7. The enrichment culture obtained at neutral pH was composed of five culturable isolates, whereas from each acidic enrichment two bacterial strains were isolated. Mesophilic isolates were Gram negative bacteria belonging to the genera Klebsiella, Pseudomonas, Stenotrophomonas and Burkholderia. Thermophilic isolates were members of the genus Bacillus. Despite the capability of the enrichment cultures to use tannic acid as single carbon and energy source, those cultures were unable to reduce the total polyphenols or the total organic carbon content of cork boiling wastewater. In order to increase the bioavailability of the organic carbon in cork boiling wastewater, biodegradation was preceded by Fenton oxidation. It was demonstrated that the combined process, using small amounts of Fenton reagents and biodegradative inoculum added almost simultaneously to cork boiling wastewater, leads to TOC reductions of more than 90%.