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Diversity of β-lactamase-encoding genes among Gram-negative isolates from water...

Manageiro, Vera; Ferreira, Eugénia; Figueira, Vânia; Manaia, Célia M.; Caniça, Manuela

Water has been recognized as a reservoir for antibiotic resistance genes (ARG), where the presence of mobile genetic elements, including plasmids, favors their dissemination. It is noteworthy that non- pathogenic environmental organisms, where plasmids encoding multiple ARG are prevalent, can provide resistance to most classes of antimicrobials including :-lactams, aminog...


Occurrence of beta-lactamases, namely GES-5 carbapenemase, among Gram-negative ...

Manageiro, Vera; Ferreira, Eugénia; Figueira, Vânia; Caniça, Manuela; Manaia, Célia M.

Antimicrobial resistant pathogens are profoundly relevant to human health and many were the studies that focused on their spread. However, natural and human associated environmental reservoirs of resistance are yet poorly understood. The main goal of this study was to evaluate the main antibiotic resistance mechanisms in Gram-negative bacteria isolates from different wastewater environments. Water samples were ...


Differential patterns of antimicrobial resistance in population subsets of Esch...

Figueira, Vânia; Serra, Elizabete; Manaia, Célia M.

The species Escherichia coli comprises different subgroups with distinct phylogeny, physiology and ecology and, thus, presumably, with different roles in antimicrobial resistance dissemination. E. coli strains isolated from raw and treated municipal wastewater and from urban water streams were characterized in terms of phylogenetic groups, antimicrobial resistance patterns and the presence of class 1 and class ...


Diversity and antibiotic resistance of aeromonas spp. in drinking and waste wat...

Figueira, Vânia; Vaz-Moreira, Ivone; Silva, Márcia; Manaia, Célia M.

The taxonomic diversity and antibiotic resistance phenotypes of aeromonads were examined in samples from drinking and waste water treatment plants (surface, ground and disinfected water in a drinking water treatment plant, and raw and treated waste water) and tap water. Bacteria identification and intra-species variation were determined based on the analysis of the 16S rRNA, gyrB and cpn60 gene sequences. Resis...


Diversity and antibiotic resistance patterns of sphingomonadaceae isolates from...

Vaz-Moreira, Ivone; Nunes, Olga C.; Manaia, Célia M.

Sphingomonadaceae (n = 86) were isolated from a drinking water treatment plant (n = 6), tap water (n = 55), cup fillers for dental chairs (n = 21), and a water demineralization filter (n = 4). The bacterial isolates were identified based on analysis of the 16S rRNA gene sequence, and intraspecies variation was assessed on the basis of atpD gene sequence analysis. The isolates were identified as members of the g...


Bacterial diversity and bioaugmentation in floodwater of a paddy field in the p...

Barreiros, Luisa; Manaia, Célia M.; Nunes, Olga C.

This work aimed at studying variations on the diversity and composition of the bacterial community of a rice paddy field floodwater, subjected to conventional management, namely by using the herbicide molinate. The promotion of the herbicide biodegradation either by the autochthonous microbiota or by a bioaugmentation process was also assessed. This study comprehended four sampling campaigns at key dates of the...


Culture-dependent and culture-independent diversity surveys target different ba...

Vaz-Moreira, Ivone; Egas, Conceição; Nunes, Olga C.; Manaia, Célia M.

Compared with culture-independent approaches, traditionally used culture-dependent methods have a limited capacity to characterizewatermicrobiota. Nevertheless, for almost a century the latter have been optimized to detect and quantify relevant bacteria. A pertinent question is if culture-independent diversity surveys give merely an extended perspective of the bacterial diversity or if, even with a higher cover...


Comparative study of the microbial diversity of bulk paddy soil of two rice fie...

Lopes, Ana R.; Faria, Cátia; Prieto-Fernández, Ángeles; Trasar-Cepeda, Carmen; Manaia, Célia M.; Nunes, Olga C.

Two adjacent paddies of an experimental rice field, subjected to organic and conventional farming, were characterized aiming the comparative assessment of microbiological variations occurring in the bulk paddy soil over the rice cycle. This study comprehended the simultaneous characterization of general physicochemical soil properties [total carbon and nitrogen, pH (H2O and KCl), C:N ratio and water content], b...


Factors influencing antibiotic resistance burden in municipal wastewater treatm...

Novo, Ana; Manaia, Célia M.

Municipal wastewater treatment plants are recognized reservoirs of antibiotic-resistant bacteria. Three municipal wastewater treatment plants differing on the dimensions and bio-treatment processes were compared for the loads of amoxicillin-, tetracycline-, and ciprofloxacinresistant heterotrophic bacteria, enterobacteria, and enterococci in the raw inflow and in the treated effluents. The sewage received by ea...


Ciprofloxacin resistance in domestic wastewater treatment plants

Manaia, Célia M.; Novo, Ana; Coelho, Bruno; Nunes, Olga C.

The potential of domestic wastewater treatment plants to contribute for the dissemination of ciprofloxacin-resistant bacteria was assessed. Differences on bacterial counts and percentage of resistance in the raw wastewater could not be explained on basis of the size of the plant or demographic characteristics of population served. In contrast, the treated effluent of the larger plants had significantly more het...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia