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Enrichment and isolation of a bacterial strain able to degrade sulfamethoxazole

Reis, P. J. M.; Reis, Ana C.; Manaia, C. M.; Corvini, Philippe F.X.; Nunes, Olga C.



Diversity and antibiotic resistance patterns of sphingomonadaceae isolates from...

Vaz-Moreira, Ivone; Nunes, Olga C.; Manaia, Célia M.

Sphingomonadaceae (n = 86) were isolated from a drinking water treatment plant (n = 6), tap water (n = 55), cup fillers for dental chairs (n = 21), and a water demineralization filter (n = 4). The bacterial isolates were identified based on analysis of the 16S rRNA gene sequence, and intraspecies variation was assessed on the basis of atpD gene sequence analysis. The isolates were identified as members of the g...


Bacterial diversity and bioaugmentation in floodwater of a paddy field in the p...

Barreiros, Luisa; Manaia, Célia M.; Nunes, Olga C.

This work aimed at studying variations on the diversity and composition of the bacterial community of a rice paddy field floodwater, subjected to conventional management, namely by using the herbicide molinate. The promotion of the herbicide biodegradation either by the autochthonous microbiota or by a bioaugmentation process was also assessed. This study comprehended four sampling campaigns at key dates of the...


Culture-dependent and culture-independent diversity surveys target different ba...

Vaz-Moreira, Ivone; Egas, Conceição; Nunes, Olga C.; Manaia, Célia M.

Compared with culture-independent approaches, traditionally used culture-dependent methods have a limited capacity to characterizewatermicrobiota. Nevertheless, for almost a century the latter have been optimized to detect and quantify relevant bacteria. A pertinent question is if culture-independent diversity surveys give merely an extended perspective of the bacterial diversity or if, even with a higher cover...


Comparative study of the microbial diversity of bulk paddy soil of two rice fie...

Lopes, Ana R.; Faria, Cátia; Prieto-Fernández, Ángeles; Trasar-Cepeda, Carmen; Manaia, Célia M.; Nunes, Olga C.

Two adjacent paddies of an experimental rice field, subjected to organic and conventional farming, were characterized aiming the comparative assessment of microbiological variations occurring in the bulk paddy soil over the rice cycle. This study comprehended the simultaneous characterization of general physicochemical soil properties [total carbon and nitrogen, pH (H2O and KCl), C:N ratio and water content], b...


Ciprofloxacin resistance in domestic wastewater treatment plants

Manaia, Célia M.; Novo, Ana; Coelho, Bruno; Nunes, Olga C.

The potential of domestic wastewater treatment plants to contribute for the dissemination of ciprofloxacin-resistant bacteria was assessed. Differences on bacterial counts and percentage of resistance in the raw wastewater could not be explained on basis of the size of the plant or demographic characteristics of population served. In contrast, the treated effluent of the larger plants had significantly more het...


Microbacterium invictum sp. nov., isolated from homemade compost

Vaz-Moreira, Ivone; Lopes, Ana R.; Faria, Cátia; Sproer, Cathrin; Schumann, Peter; Nunes, Olga C; Manaia, Célia M.

Strain DC-200T was isolated from homemade compost produced from kitchen refuse and characterized using a polyphasic approach. The isolate was a Gram-positive motile short rod, facultatively aerobic, catalase-positive and oxidase-negative, and was able to grow at 10–37 6C, pH 6.0–9.5 and with up to 5% of NaCl. The peptidoglycan was of the type B1 alpha and the muramic acid residues were glycolylated. The major f...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia