Author(s):
Justino, Jorge 
; Rauter, Amélia ; Dias, Catarina ; Martins, Alice ; Branco, Isabel ; Goulart, Margarida ; Nogueira, José ; Neng, Nuno ; Silva, Filipa ; Trevitt, Clare ; Waltho, Jon
Date: 2012
Persistent ID: http://hdl.handle.net/10400.15/882
Origin: Repositório Científico do Instituto Politécnico de Santarém
Subject(s): Salvia sclareoides; Phenolic profile; Antioxidant activity; Cytotoxicity; Genotoxicity; Prion binding
Description
Estudo de produção agronómica de espécis da salvia com vista à avaliação dos seus constituintes para o potencial controlo da doença de Alzaimer Salvia sclareoides is an aromatic herb native to Portugal, of which phenolic content (Folin–Ciocalteau method), chemical profile (HPLC/DAD), antioxidant activity (DPPH, β-carotene/linoleic acid assays), acute toxicity (MTT method, adapted for non-adherent cells), genotoxicity (short-term chromosomal aberration assay) and prion binding properties were evaluated in the acetone, water, ethanol, methanol and n-butanol extracts. The latter presented the highest phenolic content and antioxidant activity (DPPH assay), and was the single one with the flavonoids (+)-catechin, kaempferol O-glucoside and quercetin. Vanillic acid was the major component of all extracts but gallic, gentisic, caffeic, syringic, coumaric and ferulic acids were also found in some extracts. Only the n-butanol extract had components binding to the cellular form of human prion protein detected by NMR which showed specificity for two regions of the folded domain and for the unstructured N-terminal region. Extracts were not cytotoxic nor genotoxic, reinforcing the potential of S. sclareoides for nutraceutical purposes.
